Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Published on: November 2, 2014

A Do-It-Yourself and 3D-Printed Time-Lapse Imaging Platform for In-Incubator Live Cell Observation.

Yuan Yu¹, Mengyue Feng¹, Yaxin Huang²

¹School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine; Reproductive & Women-Children Hospital, Chengdu University of Traditional Chinese Medicine.

Journal of Visualized Experiments : Jove

|June 1, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Icariin in male reproduction: Multi-target pharmacological mechanisms and therapeutic potential.

European journal of pharmacology·2026

Same author

BMAL1 modulates respiratory syncytial virus-induced inflammation through p38 mitogen-activated protein kinase pathway: insights into circadian regulation.

Microbial pathogenesis·2025

Same author

A novel model of central precocious puberty disease: Paternal MKRN3 gene-modified rabbit.

Animal models and experimental medicine·2025

Same author

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits.

Journal of visualized experiments : JoVE·2025

Same author

Bisphenol A impairs oocyte maturation by dysfunction of cumulus cells.

Theriogenology·2024

Same author

Adulthood bisphenol A exposure induces anxiety in male mice via downregulation of alpha-1D adrenergic receptor in paraventricular thalamus.

Ecotoxicology and environmental safety·2023

This summary is machine-generated.

Researchers developed a low-cost, 3D-printed time-lapse imaging platform for incubators. This do-it-yourself system offers customizable, high-resolution live-cell and embryo imaging, overcoming limitations of expensive commercial options.

Area of Science:

Biotechnology
Microscopy
Cell Biology

Background:

Commercial in-incubator microscopy systems are expensive and proprietary, limiting accessibility.
Existing open-source alternatives often compromise imaging performance or adaptability.

Purpose of the Study:

To develop a low-cost, customizable, do-it-yourself (DIY) time-lapse imaging platform for use inside standard cell culture incubators.
To provide a reproducible and accessible solution for long-term live-cell and embryo imaging.

Main Methods:

Designed and constructed a 3D-printed imaging platform controlled by a Raspberry Pi.
Integrated stepper motor actuation and a programmable LED backlight for bright-field and oblique illumination.
Utilized open-source Python software for automated multi-well imaging and scheduled time-lapse acquisition.

More Related Videos

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Published on: November 2, 2014

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Main Results:

The platform demonstrated reliable long-term imaging of cultured cells and early embryos under incubator conditions.
Achieved an optical resolution of approximately 1.55 µm using a USAF 1951 target.
Exhibited low drift, high repeatability, and accurate positioning.

Conclusions:

The DIY platform offers a cost-effective, reproducible, and customizable alternative to commercial systems.
The system is adaptable for various plate formats and specialized culture setups.
Enables accessible, high-quality live-cell and embryo imaging in research laboratories.