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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Three-Dimensional Microscopy in Microbiology01:28

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 3, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Parallel image-scanning autocorrelation-deconvolution microscopy.

Caini Xiao, Xiaoyuan Du, Wenfeng Fu

    Optics Letters
    |June 1, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Parallel image-scanning autocorrelation-deconvolution microscopy (PISADM) enhances super-resolution imaging by improving the separation of closely spaced structures. This new method achieves higher resolution and better structural fidelity in dense biological samples.

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    Last Updated: Jun 3, 2026

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
    07:12

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

    Published on: January 6, 2026

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
    06:51

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

    Published on: August 2, 2018

    Area of Science:

    • Microscopy
    • Super-resolution imaging
    • Biophotonics

    Background:

    • Super-resolution microscopy aims to overcome the diffraction limit for detailed cellular imaging.
    • Reconstruction stability and separability of dense structures remain challenges in current super-resolution techniques.

    Purpose of the Study:

    • To introduce and validate a novel multifocal-scanning strategy, parallel image-scanning autocorrelation-deconvolution microscopy (PISADM).
    • To enhance the resolution and separability of closely spaced structures in super-resolution imaging.

    Main Methods:

    • PISADM employs spatially localized multifocal excitation and scanning-position-wise autocorrelation-deconvolution (SACD) reconstruction.
    • Focus-wise pixel reassignment is utilized to reduce cross-talk and improve reconstruction stability.
    • Monte-Carlo simulations and Fourier ring correlation analysis were used for quantitative evaluation.

    Main Results:

    • PISADM reduced the stable separability limit to ~100-110 nm, compared to ~140-150 nm for standard SACD at 10 dB SBR.
    • The method preserves reliable high-frequency information near the separability limit.
    • Improved separation of neighboring microtubules was observed in 2D, long-term, and 3D imaging with enhanced structural fidelity.

    Conclusions:

    • PISADM offers a practical approach for resolving densely packed structures with small spatial separations.
    • The technique significantly improves resolution and stability in super-resolution microscopy.
    • PISADM demonstrates superior performance in imaging complex biological structures like microtubules.