Related Experiment Video
Updated: Jun 3, 2026

High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato
Published on: June 7, 2024
Spring low temperature stress reduces wheat yield by accelerating post-anthesis flag leaf senescence via ROS
Xiang Chen1, Weixiang Huang1, Li Yang1
1College of Agriculture, Anhui Agricultural University, National Engineering Laboratory of Crop Stress Resistance Breeding, Institute of Hefei Artificial Intelligence Breeding Accelerator Co. Ltd, Huaibei Field Comprehensive Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Hefei, 230036, Anhui, China.
None:
Spring low-temperature stress (SLTS) poses a major threat to wheat (Triticum aestivum L.) productivity under increasing climate global warming, but its post-anthesis physiological legacy effects remain insufficiently understood. A two-year experiment was conducted using the SLTS-resistant cultivar Yannong19 (YN) and the SLTS-susceptible cultivar Xinmai26 (XM), which were exposed to 10 °C (CK), 2 °C (T1), and -2 °C (T2) at the anther differentiation stage. Post-anthesis changes in flag leaf senescence, SPAD values, antioxidant metabolism, reactive oxygen species accumulation, and grain yield were evaluated. Compared with CK, SLTS accelerated flag leaf senescence, decreased SPAD values, and reduced grain yield by 9%-25% under T1 and 25%-42% under T2, with greater losses in XM than in YN. SLTS also decreased soluble sugar, soluble protein, and glutathione (GSH) contents, as well as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, whereas hydrogen peroxide (H2O2) and malondialdehyde (MDA) accumulated, especially under T2 and in XM. Random forest analysis indicated that yield variation was mainly associated with early-stage POD activity and SPAD values, followed by late-stage H2O2 and GSH contents. These findings suggest that SLTS-induced disruption of antioxidant homeostasis is associated with accelerated post-anthesis flag leaf senescence and yield loss, providing insight into the physiological legacy effects of SLTS in wheat.
Related Concept Videos
Responses to Heat and Cold Stress
Responses to Drought and Flooding
Responses to Salt Stress
Adaptations that Reduce Water Loss
Regulation of Transpiration by Stomata
Other Stress Responses in Bacteria
