Synthetic progress and applications of bioaffinity chromatography columns

Bioaffinity chromatography (BAC) is a highly selective separation technique based on specific biological recognition. With the outstanding specificity of the affinity chromatography column, BAC plays an irreplaceable role in a series of detection technologies, including drug target identification, proteomics analysis, and trace substance detection in complex matrices. In recent years, with the development of chromatographic column synthesis technology, the core technology of BAC has made significant breakthroughs. Recent advances in chromatographic column synthesis have witnessed significant breakthroughs in the core technology of BAC, and it has been widely applied in analytical research. The stability, immobilization efficiency, and durability of its chromatographic column have been greatly improved, and the analysis cost has also been significantly reduced. Therefore, it is widely used in analytical research. In the field of drug analysis, this technique demonstrates unique advantages in separating drug targets in complex matrices, detecting biomarkers, and studying drug-receptor interactions, thanks to its ultra-high selectivity. This paper is based on theories of BAC developed since the 21st century, and also references some representative articles from the end of the last century as its theoretical background and focused on analyzing the development and innovation of each component of BAC during the period from 2016 to 2026, and commented on its development and innovative applications in four directions in drug analysis: enrichment and initial purification of macromolecular substances, enrichment of trace substances in complex matrices, separation of chiral drugs, and detection and identification of active substances in complex components aiming to provide a reference for in-depth research in the relevant field.