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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

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Related Experiment Video

Updated: Jun 3, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Synthetic progress and applications of bioaffinity chromatography columns.

Changming Tang1, Qinghua Yue1, Longshan Zhao1

  • 1School of Pharmacy, Shenyang Pharmaceutical University, Benxi 117004, China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|June 1, 2026
PubMed
Summary

Bioaffinity chromatography (BAC) offers superior specificity for drug analysis and biomolecule separation. Recent innovations in column technology have enhanced its efficiency, stability, and cost-effectiveness for complex sample analysis.

Keywords:
ApplicationsBioaffinity chromatographyDrug analysis

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Last Updated: Jun 3, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
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Published on: August 16, 2019

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chromatography

Background:

  • Bioaffinity chromatography (BAC) is a highly selective separation technique utilizing specific biological recognition.
  • Its specificity makes it crucial for drug target identification, proteomics, and trace substance detection.
  • Recent advancements in chromatographic column synthesis have significantly improved BAC's core technology.

Purpose of the Study:

  • To analyze the development and innovation of BAC components from 2016 to 2026.
  • To comment on BAC's innovative applications in drug analysis.
  • To provide a reference for in-depth research in relevant fields.

Main Methods:

  • Review of theoretical developments in BAC since the 21st century.
  • Inclusion of representative articles from the late 20th century for theoretical background.
  • Focus on analyzing component innovations and applications in drug analysis.

Main Results:

  • Significant breakthroughs in BAC core technology, enhancing column stability, immobilization efficiency, and durability.
  • Reduced analysis costs associated with BAC.
  • Wide application of BAC in analytical research, particularly in drug analysis.

Conclusions:

  • BAC demonstrates unique advantages in drug analysis, including separating targets, detecting biomarkers, and studying drug-receptor interactions.
  • Innovative applications include enrichment of macromolecules and trace substances, chiral drug separation, and active substance identification.
  • BAC is a valuable tool for complex sample analysis and drug discovery.