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Related Experiment Video

Updated: Jun 3, 2026

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
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Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

Published on: March 19, 2021

Rapid neuronal labeling and functional imaging in the developing mouse brain with AAV-PHP.eB.

Weiyi Ye1, Mao Deng1, Sijing Zhao2

  • 1Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China.

Cell Reports Methods
|June 1, 2026
PubMed
Summary
This summary is machine-generated.

Early postnatal delivery of adeno-associated virus (AAV)-PHP.eB in mice enables rapid neuronal labeling and functional imaging. This method facilitates high-resolution structural reconstruction and longitudinal studies of developing neural circuits.

Keywords:
AAV-PHP.eBCP: imagingCP: neuroscienceGCaMPcalcium imagingdeveloping brainsingle-neuron labeling

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Last Updated: Jun 3, 2026

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Published on: March 13, 2016

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Neuronal labeling is crucial for understanding neural circuit development.
  • Adeno-associated virus (AAV) gene delivery is a versatile tool, but its efficacy in the developing brain requires further investigation.

Purpose of the Study:

  • To determine the effectiveness of AAV-PHP.eB for neuronal labeling in the neonatal mouse brain.
  • To establish a method for high-resolution structural and functional imaging of developing neural circuits.

Main Methods:

  • Administration of AAV-PHP.eB in neonatal mice.
  • Titration of AAVs for sparse neuronal labeling.
  • Delivery of GCaMP for functional imaging.
  • Longitudinal in vivo two-photon calcium imaging.

Main Results:

  • Neuronal transduction observed as early as 3 days post-injection.
  • Achieved sparse labeling for high-resolution dendritic morphology reconstruction.
  • Enabled longitudinal in vivo functional imaging in the immature brain.

Conclusions:

  • AAV-PHP.eB-mediated early postnatal delivery is a rapid, efficient, and minimally invasive method for neuronal labeling and functional imaging in the developing brain.
  • This approach provides new opportunities to study neural circuit development at structural and functional levels.