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Updated: Jun 3, 2026
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
Published on: March 12, 2021
Integrated RNA-seq and ATAC-seq analysis of miR-101 in bovine somatic cell reprogramming
Deji Luan1, Yile Wang1, Ying Wang2
1College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, China.
Genomics
|June 1, 2026
Summary
Overexpressing miR-101 in somatic cell nuclear transfer (SCNT) donor cells improves cloned embryo development. This research identifies key genes involved in early nuclear reprogramming, enhancing SCNT efficiency.
Area of Science:
- Animal biotechnology
- Reproductive science
- Epigenetics
Background:
- Somatic cell nuclear transfer (SCNT) faces challenges like low efficiency and developmental issues.
- Incomplete reprogramming and epigenetic modifications are linked to suboptimal SCNT outcomes.
- MicroRNA-101 (miR-101) is implicated in embryonic development.
Purpose of the Study:
- To investigate the role of miR-101 in enhancing SCNT efficiency.
- To identify genes and pathways involved in early nuclear reprogramming mediated by miR-101.
- To establish stable cell lines overexpressing miR-101 for SCNT applications.
Main Methods:
- Construction and validation of a miR-101 overexpression vector (miR-101-CD-513B1).
- Generation of stable monoclonal bovine fetal fibroblast cell lines with elevated miR-101 levels.
- RNA-sequencing (RNA-seq) and ATAC-sequencing (ATAC-seq) analyses.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.
Main Results:
- Stable cell lines overexpressing miR-101 were successfully established.
- miR-101 overexpression led to increased open chromatin regions, suggesting altered epigenetic landscapes.
- RNA-seq identified candidate genes associated with reprogramming.
- Target gene prediction and pathway analysis revealed genes linked to early nuclear reprogramming in SCNT embryos.
Conclusions:
- Overexpression of miR-101 enhances the developmental potential of nuclear transfer embryos.
- miR-101 plays a crucial role in regulating early nuclear reprogramming processes in SCNT.
- This study provides insights into improving SCNT efficiency through epigenetic modulation.
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