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Updated: Jun 3, 2026

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
Published on: October 17, 2017
Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome
Lan-Ying Lee1,2, Yunjia Shen1,2, Yooyoung Kim1,2
1Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907-1392, USA.
Abstract:
Agrobacterium transfer DNA (T-DNA) integration is mediated by plant DNA repair proteins of various nonhomologous end-joining (NHEJ) pathways. However, the relative importance of these proteins for stable transformation of Arabidopsis thaliana is controversial. Using quantitative transient and stable transformation assays and droplet digital PCR analyses, we characterized single and higher order Arabidopsis NHEJ mutants to determine the importance of numerous NHEJ proteins in transformation and T-DNA integration. Simultaneous mutation of genes important for several DNA repair/recombination pathways may only partially inhibit stable Agrobacterium-mediated transformation. Some of these mutations may also inhibit transient transformation. These observations support the hypothesis that no individual NHEJ protein is essential for T-DNA integration.
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