Related Experiment Video
Updated: Jun 3, 2026

Exploring the Longissimus Muscle: Unraveling its Correlation with Meat Quality in Bos indicus and Crossbred Bulls
Published on: July 12, 2024
Beef Color Standard, including marbled Wagyu beef, determined using a portable near-infrared optical fiber
Kazunori Matsumoto1, Naoaki Obana2, Masakazu Irie2
1National Livestock Breeding Center, Nishigo, Japan. k0matsumoto@nlbc.go.jp.
Objective:
The purpose of this study was to see if a portable near-infrared optical fiber spectrometer with a wavelength range of 700-1,050 nm could objectively predict the Beef Color Standard (BCS), which is visually evaluated during beef carcass grading.
Methods:
A total of 1,077 carcasses were procured from Japanese Black (JB) Wagyu (n = 534), crossbred (F1) (n = 367), and dairy cows (n = 176) shipped to meat markets. The cut surface between the sixth and seventh ribs was visually evaluated by grading members using the BCS. In addition, a near-infrared optical fiber spectrometer was applied to the cut surface to obtain the reflectance spectrum. Using data from 827 carcasses, a calibration model was established through a partial least squares regression analysis using the BCS value as the objective variable, while the optical data following quadratic differential processing as the explanatory variable. Using data from 250 additional animals, the calibration model and predicted BCS values were verified for accuracy.
Results:
The calibration model exhibited a high correlation coefficient of 0.83 and a standard error of calibration of 0.70. The predicted BCS by near-infrared evaluation rarely differed significantly from the actual BCS, and the difference was roughly distributed in the range of ±1. For verifying the accuracy of our calibration model, the correlation coefficient between the actual BCS and the predicted value for the validation sample was 0.75, and the standard error of prediction was 0.71. The probability that the error between the measured and optically predicted BCS values in the validation samples was within ±1 and ±0 was 97.6% and 54.4%, respectively.
Conclusion:
Using a portable near-infrared fiber optic spectrometer, we demonstrated that the BCS value of beef carcasses, particularly marbled Wagyu beef in meat markets, can be objectively evaluated with relatively high accuracy.
More Related Videos
Related Concept Videos
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectroscopy: Beer–Lambert Law
UV–Vis Spectroscopy: Woodward–Fieser Rules
IR Spectrometers
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

