Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylglyoxal Scavengers Resensitize KRAS-Mutated Colorectal Tumors to Cetuximab.

Cell reports·2026
Same author

Response to: Comment on "Plasma-based low-grade inflammation and endothelial dysfunction explain a small but significant proportion of the association between the n-3 polyunsaturated fatty acids and cardiovascular diseases: The Maastricht Study".

Journal of clinical lipidology·2026
Same author

Plasma-based low-grade inflammation and endothelial dysfunction explain a small but significant proportion of the association between the n-3 polyunsaturated fatty acids and cardiovascular diseases: The Maastricht Study.

Journal of clinical lipidology·2026
Same author

<i>Trans</i>-eQTLs reveal the architecture of human gene regulatory networks.

medRxiv : the preprint server for health sciences·2026
Same author

Higher intake of dietary dicarbonyl compounds is associated with lower incidence of type 2 diabetes: European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct case-cohort study.

European journal of nutrition·2026
Same author

Metabolite Erythritol in 24-Hour Urine Is Associated With Higher Intrahepatic Lipid Content: The Maastricht Study.

Diabetes·2026

Related Experiment Video

Updated: Jun 3, 2026

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
07:32

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

Managing Autofluorescence in Spectral Flow Cytometry for Macrophage Identification in the Liver.

Sabine Daemen1, Tuneille I Adelaar1, Panos Barlampas1,2,3,4

  • 1Department of Internal Medicine, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

European Journal of Immunology
|June 2, 2026
PubMed
Summary

Autofluorescence (AF) in biological tissues complicates flow cytometry analysis. Spectral flow cytometry helps correct AF, improving macrophage subset analysis in healthy and MASH murine livers.

Keywords:
autofluorescencelivermacrophagesmetabolic dysfunction‐associated steatohepatitis (MASH)spectral flow cytometry

More Related Videos

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
06:22

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

Related Experiment Videos

Last Updated: Jun 3, 2026

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
07:32

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
06:22

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

Area of Science:

  • Immunology
  • Biotechnology
  • Pathology

Background:

  • Autofluorescence (AF) from biological tissues complicates flow cytometry by distorting signals.
  • Spectral flow cytometry can visualize and extract AF, but correction remains challenging in complex samples like the liver.
  • Liver diseases alter tissue composition and macrophage AF properties, necessitating advanced analytical methods.

Purpose of the Study:

  • To apply spectral flow cytometry for improved analysis of macrophage subpopulations in healthy and metabolic dysfunction-associated steatohepatitis (MASH) murine livers.
  • To investigate the impact of autofluorescence on macrophage quantification and subset differentiation in liver disease models.

Main Methods:

  • Utilized spectral flow cytometry to analyze macrophage populations in healthy, steatotic, and MASH murine livers.
  • Developed and applied strategies for autofluorescence extraction and correction in complex hepatic tissues.

Main Results:

  • Healthy, steatotic, and MASH livers displayed distinct and heterogeneous autofluorescence signatures.
  • Inadequate autofluorescence extraction led to inaccurate quantification and differentiation of hepatic macrophage subsets.
  • Macrophages exhibit significant AF heterogeneity, particularly in MASH models.

Conclusions:

  • Spectral flow cytometry is crucial for accurate AF correction in liver research.
  • Proper AF management is essential for reliable analysis of hepatic macrophage heterogeneity in health and disease.
  • Understanding AF signatures aids in dissecting macrophage roles in liver pathophysiology.