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Updated: Jun 3, 2026
09:43
Breeding by Design for Functional Rice with Genome Editing Technologies
Published on: January 3, 2025
OsBBX19 encoding a CCT domain-containing protein controls heading date in rice.
Qinqin Yang1, Siliang Xu1, Peng Xu1
1State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006 China.
Summary
The rice gene OsBBX19 regulates heading date by influencing flowering pathways and interacting with CPIP1. Its variations are linked to indica rice improvement, offering insights for breeding optimization.
Area of Science:
- Plant Biology
- Genetics
- Agronomy
Background:
- Heading date is crucial for rice adaptation and yield.
- OsBBX19 (OsCCT22) is a known regulator of rice heading date.
Purpose of the Study:
- To perform molecular functional characterization of OsBBX19.
- To elucidate the role of OsBBX19 in rice flowering time regulation.
Main Methods:
- Overexpression of OsBBX19 in rice.
- GUS staining and RT-qPCR for gene expression analysis.
- Protein-protein interaction assays (OsBBX19 with CPIP1).
- Haplotype analysis of OsBBX19.
Main Results:
- OsBBX19 overexpression delayed heading under short and long-day conditions.
- OsBBX19 negatively regulates Hd3a, RFT1, and Ehd1, and positively regulates Ghd7.
- OsBBX19 physically interacts with CONSTANS-like protein interacting protein 1 (CPIP1).
- Significant indica-japonica differentiation in OsBBX19 haplotypes was observed, with increased frequency of Hap3 and Hap5 during indica rice improvement.
Conclusions:
- OsBBX19 functions within the Ghd7-Ehd1-Hd3a/RFT1 flowering pathway.
- OsBBX19's interaction with CPIP1 is demonstrated.
- OsBBX19 genetic variations are associated with rice breeding progress, particularly in indica varieties.
- OsBBX19 serves as a potential genetic resource for optimizing rice heading date and yield.
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