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Updated: Jun 3, 2026
Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
Published on: December 2, 2022
IL-8 is a potential biomarker for retinal detachment secondary to choroidal melanoma
Zhen Xing1, Xinran Zhang1, Wei Yu1
1Department of Ophthalmology, The Affiliated Hospital of Southwest Medical University, Sichuan, China.
Abstract:
This study aims to identify potential biomarkers for retinal detachment secondary to choroidal melanoma (CM). Mendelian randomisation (MR) analysis, immunohistochemistry, cell proliferation and migration assays, co-culture experiments involving human microglial cells (HMC3) and CM cells, and tube formation assay were employed to validate the role of IL-8 in retinal detachment secondary to choroidal melanoma. Mendelian randomisation analysis identified four inflammatory mediators causally linked to malignant melanoma progression: CDCP1 concentration (P = 0.017, OR = 0.999, 95% CI [0.998-1.000]), CSF-1 concentration (P = 0.020, OR = 1.002, 95% CI [1.000-1.003]), IL-10Rβ concentration (P = 0.004, OR = 0.999, 95% CI [0.998-1.000]), IL-17C concentration (P = 0.002, OR = 1.003, 95% CI [1.001-1.005]); Four inflammatory factors exhibited a causal relationship with retinal detachment progression: IL-15Rα concentration (P = 0.029, OR = 1.001, 95% CI [1.000-1.001]), IL-2Rβ concentration (P = 0.007, OR = 1.003, 95% CI [1.001-1.004]), IL-8 concentration (P = 0.045, OR = 1.002, 95% CI [1.000-1.004]), TSLP concentration (P = 0.028, OR = 1.002, 95% CI [1.000-1.004]). MR analysis indicated that genetically predicted IL-8 levels are associated with an increased risk of retinal detachment. IL-8 is highly expressed in CM tissues and promotes the proliferation of CM and HMC3 cells. It partially relieves the inhibitory effects mediated by the supernatant of HMC3 cells and promotes angiogenesis. IL-8 may be involved in CM-related inflammatory microenvironments and secondary exudative retinal detachment. It is a potential biomarker and provides a new target for targeted anti-inflammatory therapy and improved patient prognosis.
Insights
Interleukin-8 (IL-8) is identified as a key biomarker for retinal detachment secondary to choroidal melanoma (CM). This study highlights IL-8's role in promoting cell proliferation and angiogenesis, suggesting it as a target for anti-inflammatory therapies.
Area of Science:
- Ophthalmology
- Oncology
- Immunology
Background:
- Choroidal melanoma (CM) can lead to secondary retinal detachment, a serious complication.
- Identifying biomarkers for this condition is crucial for early detection and treatment.
- The inflammatory microenvironment plays a significant role in CM progression and associated retinal detachment.
Purpose of the Study:
- To identify potential biomarkers for retinal detachment secondary to choroidal melanoma (CM).
- To investigate the role of Interleukin-8 (IL-8) in the development of CM-related retinal detachment.
- To explore IL-8 as a potential therapeutic target.
Main Methods:
- Mendelian randomisation (MR) analysis to identify causal inflammatory mediators.
- Immunohistochemistry to assess IL-8 expression in CM tissues.
- Cell proliferation, migration, and tube formation assays to evaluate IL-8 function.
- Co-culture experiments with human microglial cells (HMC3) and CM cells.
Main Results:
- MR analysis identified several inflammatory mediators linked to CM progression and retinal detachment.
- Genetically predicted IL-8 levels showed an association with increased risk of retinal detachment.
- IL-8 was highly expressed in CM tissues and promoted proliferation of CM and HMC3 cells.
- IL-8 promoted angiogenesis and partially overcame inhibitory effects from microglial cell supernatant.
Conclusions:
- IL-8 is implicated in the inflammatory microenvironment of CM and contributes to secondary exudative retinal detachment.
- IL-8 serves as a potential biomarker for CM-related retinal detachment.
- Targeted anti-inflammatory therapy focusing on IL-8 may improve patient prognosis.
