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Comparative Analysis of Two Consecutive Genome Sequencing Results of Enterococcus Faecium Strain Entfacye
Yazdanizad Maryam1, Saboor Yaraghi Ali Akbar2, Javdani Shahedin Golshid3
1Department of Medical Biotechnology, TMS.C., Islamic Azad University, Tehran, Iran.
Introduction:
The overuse of antimicrobials in healthcare has driven the emergence, persistence, and rapid spread of antimicrobial-resistant pathogens. Vancomycin-resistant enterococci (VRE), mainly Enterococcus faecium, have recently emerged as multi drug-resistant (MDR) bacteria worldwide. Consequently, enterococcal infections have become more challenging to treat due to their increased multiple-drug resistance. Studying the genome of an enterococcal isolate and investigating genome changes over time help researchers better understand the development of antimicrobial resistance in bacterial isolates.
Materials & Methods:
In the present study, E. faecium EntfacYE isolate from a human biological sample was assessed. After phenotypic, biochemical,and molecular verifications of the bacterial isolate, the bacterial genome was completely sequenced.
Results:
In total, the EntfacYE genomic subsystems contained 23 various categories with 46 antimicrobial resistance genes. In the current study, 31 antimicrobial resistance genes were reported in the subsystems, and 15 genes had no subsystems. Genes conferring tetracycline resistance were reported in this study. Increases in the number and type of antimicrobial resistance genes were recorded, indicating that bacteria are becoming rapidly resistant to available antimicrobials.
Conclusion:
In general, the study of antimicrobial resistance genes in bacteria can be effective in better understanding resistance patterns and mechanisms, which can lead to find novel protocols for limiting spread of antimicrobial resistance in bacteria.
Insights
Antimicrobial resistance is a growing threat. This study sequenced the genome of an Enterococcus faecium isolate, revealing numerous antimicrobial resistance genes and highlighting the rapid evolution of bacterial resistance.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Overuse of antimicrobials drives antimicrobial resistance.
- Vancomycin-resistant Enterococci (VRE) are a significant global health concern.
- Understanding bacterial resistance mechanisms is crucial for developing new treatments.
Purpose of the Study:
- To sequence and analyze the genome of an Enterococcus faecium isolate (EntfacYE).
- To identify antimicrobial resistance genes within the bacterial genome.
- To understand the genomic basis of multi-drug resistance in E. faecium.
Main Methods:
- Phenotypic, biochemical, and molecular characterization of the E. faecium isolate.
- Whole-genome sequencing of the EntfacYE isolate.
- Analysis of genomic subsystems and antimicrobial resistance genes.
Main Results:
- The EntfacYE genome contained 46 antimicrobial resistance genes across 23 genomic subsystems.
- 31 resistance genes were identified within subsystems, with 15 lacking subsystem classification.
- Tetracycline resistance genes were identified, alongside an increase in the number and types of resistance genes.
Conclusions:
- Genomic analysis of bacterial isolates aids in understanding antimicrobial resistance.
- Identifying resistance genes and patterns can inform strategies to combat resistance.
- This study contributes to understanding the rapid evolution of antimicrobial resistance in bacteria.
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