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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

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Published on: February 17, 2023

Establishing efficient multi-gene editing tools for papaya.

Bowei Wang¹, Xuesong Cao^2,3, Zeng Lin¹

¹Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

Horticulture Research

|June 2, 2026

Summary

This summary is machine-generated.

Researchers developed a new papaya hairy root system to speed up gene editing. This system enables efficient base editing (ABE/CBE) and precision breeding for improved papaya crops.

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Area of Science:

Agricultural Science
Plant Biotechnology
Genomics

Background:

Papaya genetic improvement is hindered by traditional, time-consuming tissue culture methods.
Gene editing in papaya lags behind other crops due to these breeding challenges.

Purpose of the Study:

To develop a tissue culture-independent hairy root system for efficient papaya gene editing.
To establish and validate adenine base editor (ABE) and cytosine base editor (CBE) tools in papaya.

Main Methods:

Developed a novel hairy root system, bypassing traditional tissue culture.
Pre-tested sgRNA activity and achieved high editing efficiency for the CpWIP3 gene.
Conducted promoter screening to develop ABE and CBE tools.

Main Results:

Successfully established a tissue culture-independent platform for papaya gene editing.
Demonstrated high editing efficiency for CpWIP3 using the new system.
Developed the first precise single-nucleotide editing system (ABE and CBE) for papaya.

Conclusions:

The hairy root system significantly accelerates gene editing applications in papaya.
This platform facilitates functional genomics and precision breeding in papaya.
Enables pre-assessment of editing efficiency, reducing costs and failure rates.