Related Experiment Video
Updated: Jun 3, 2026
07:03
In Vitro Modeling of Fat Deposition in Metabolic Dysfunction-Associated Steatotic Liver Disease
Published on: July 19, 2024
Precision pathophysiology in steatotic liver disease
Wonseok Lee1, Da Kyung Hwang2, Hyun Young Kim3,4
1College of Pharmacy, Gachon University, Incheon, Republic of Korea.
Clinical and Molecular Hepatology
|June 2, 2026
Summary
Steatotic liver disease (SLD) encompasses metabolic dysfunction-associated steatotic liver disease (MASLD) and alcohol-associated liver disease (ALD). This review explores their overlap and distinctions, aiding in targeted therapy development.
Area of Science:
- Hepatology and metabolic disease research.
- Integrative multi-omics and cellular biology.
Background:
- Steatotic liver disease (SLD) includes metabolic dysfunction-associated steatotic liver disease (MASLD) and alcohol-associated liver disease (ALD), with overlapping causes.
- MASLD involves SLD with cardiometabolic dysfunction; MetALD is MASLD with moderate alcohol intake.
- Significant overlap between MASLD and MetALD necessitates understanding their combined pathological and molecular impacts.
Purpose of the Study:
- To review the pathophysiology of SLD, differentiating MASLD and MetALD.
- To discuss experimental models for studying these liver diseases.
- To accelerate the development of targeted therapies for SLD.
Main Methods:
- Review of current literature on SLD, MASLD, and MetALD.
- Analysis of multi-omic technologies for single-cell profiling (genetic, epigenetic, spatial, proteomic).
- Examination of experimental models, including human liver spheroids.
Main Results:
- SLD comprises MASLD, MetALD, and ALD with shared etiologies.
- MASLD and MetALD show considerable phenotypic overlap despite distinct classifications.
- Multi-omic approaches offer high-resolution insights into cellular heterogeneity and disease mechanisms.
Conclusions:
- Understanding the distinctions and overlaps between MASLD and MetALD is crucial.
- Advanced profiling technologies and experimental models enhance insights into SLD.
- This knowledge facilitates the development of precise therapeutic strategies for liver disorders.
Related Concept Videos
01:24Cirrhosis II: Pathophysiology
Cirrhosis is a progressive chronic liver injury caused by prolonged inflammation, excessive fibrotic remodeling, and impaired regeneration. Over time, repeated hepatic insults disrupt the liver’s architecture and function, leading to reduced blood flow, impaired bile drainage, and diminished metabolic capacity.Pathophysiology of cirrhosisCirrhosis arises from three main responses to chronic liver damage: inflammation, immune activation, and hepatocyte death. These processes lead to structural...
01:21Chronic Pancreatitis II: Pathophysiology
Chronic pancreatitis is a progressive and irreversible inflammation of the pancreas, most often caused by long-term alcohol abuse, but it can also be related to ductal obstruction, smoking, or genetic factors.Chronic pancreatitis occurs when the pancreas is repeatedly exposed to harmful agents like alcohol, smoking, ductal obstruction, or genetic predisposition. These factors lead to the release of toxic metabolites and inflammatory cytokines, sustaining chronic inflammation in the pancreatic...
01:22Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test
In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess the...
01:23Cirrhosis I: Introduction
Cirrhosis is a chronic, irreversible liver disease characterized by the widespread replacement of healthy liver tissue with fibrotic scar tissue and the formation of regenerative nodules.Etiology of cirrhosisCirrhosis results from sustained liver injury that triggers progressive fibrosis and structural remodeling. The underlying causes are diverse, encompassing common and less frequent clinical conditions. Regardless of the origin, all causes lead to chronic inflammation, hepatocyte loss, and...
01:24Chronic Pancreatitis I: Introduction
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
01:25Chronic Pancreatitis I: Introduction
Chronic pancreatitis is a long-standing, relapsing inflammation of the pancreas, characterized by irreversible damage to the gland. It results in progressive destruction of the pancreatic parenchyma, fibrosis, and eventual loss of both exocrine and endocrine function. The disease may evolve gradually after multiple episodes of acute pancreatitis or develop independently.EtiologyChronic pancreatitis can arise from a variety of causes:Alcohol use is the leading cause, accounting for 70–80% of...