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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.

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Updated: Jun 3, 2026

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

Harnessing CRISPR-Cas Technology for Precision Antimicrobial Targeting.

Zulfa Nooreen¹, Neha Verma¹, Shailendra Singh Narwariya²

¹PSIT-Pranveer Singh Institute of Technology (Pharmacy) NH19 Bhaunti Kanpur, India.

Current Topics in Medicinal Chemistry

|June 2, 2026

Summary

CRISPR-Cas technology offers a precise method to combat antimicrobial resistance (AMR) by targeting specific genes in pathogens. This innovative approach effectively eliminates harmful bacteria and viruses while preserving beneficial microbes, transforming infection control.

Keywords:

Antimicrobial resistance Bacteriophage CRISPR-Cas system ESKAPE Gene editing

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Last Updated: Jun 3, 2026

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

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Published on: October 14, 2025

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

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Published on: August 14, 2021

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Area of Science:

Microbiology
Genetics
Biotechnology

Background:

Misuse of antibiotics has led to a rise in antimicrobial resistance (AMR), a significant global health threat posed by pathogens like ESKAPE.
Traditional antibiotics are ineffective against resistant microbes, necessitating novel therapeutic strategies.
CRISPR-Cas systems offer a precise method for targeting specific bacterial genes to combat AMR.

Purpose of the Study:

To review the application of CRISPR-Cas systems in combating antimicrobial resistance.
To highlight the potential of CRISPR-Cas technology as a precision antimicrobial agent.
To explore advancements in delivery systems for CRISPR-Cas applications.

Main Methods:

Literature review of publications from 2015-2024 focusing on CRISPR-Cas systems and AMR.
Inclusion of keywords such as antimicrobial resistance, CRISPR-Cas system, and ESKAPE.
Gathering information from various online sources and review papers.

Main Results:

CRISPR-Cas systems show promise in targeting AMR bacteria and viruses, especially with advanced delivery systems.
The technology can selectively degrade foreign DNA, leading to bacterial death or suppression of virulence factors.
CRISPR has demonstrated efficacy against drug-resistant bacteria and viruses like SARS-CoV-2 and Hepatitis B.

Conclusions:

CRISPR-Cas technology provides a precise, tailored approach to combat AMR by targeting specific resistance and virulence genes.
It offers efficient eradication of infections with minimal impact on beneficial bacteria, unlike conventional antibiotics.
Advancements in delivery systems enhance its capability against diverse resistant pathogens, potentially revolutionizing infection control.