Related Experiment Video
Updated: Jun 3, 2026

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension
Published on: May 17, 2024
In vivo Antihypertensive Activity of Enzyme Hydrolysates from Red Tilapia (Oreochromis spp.) Viscera
Lorena Arias1, Claudia P Sánchez-Henao1, José E Zapata1
1Nutrition and Food Technology Research Group, Pharmaceutical and Food Sciences Faculty, Universidad de Antioquia, Medellín 050010, Colombia.
Introduction/Objective:
This study aimed to evaluate the in vitro and in vivo antihypertensive effects of red tilapia (Oreochromis spp.) viscera hydrolysate and its fractions.
Methods:
The viscera were hydrolyzed by Alcalase® 2.4L, and their fractions were separated in a ceramic tubular membranes system with an MWCO of 3 kDa, obtaining >3 kDa and <3 kDa fractions. Both hydrolysate and fractions were evaluated for vasodilatory effect in rat aorta rings and in an acute test involving normotensive rats. In addition, the chronic antihypertensive effect of the fraction <3 kDa was evaluated in hypertensive rats for 35 days, using doses of 75 and 150 mg/kg, respectively.
Results:
The hydrolysate induced vasodilation in rat aorta rings through an endotheliumdependent nitric oxide (NO) pathway. In the acute in vivo test, hydrolysates and fractions significantly decreased rat systolic pressure, with the <3 kDa fraction showing the most potent effect.
Discussion:
Meanwhile, in the chronic antihypertensive study with rats, a blood pressure drop was observed over time, reducing systolic blood pressure to 122.7 mmHg on day 29 of the study, using the fraction <3 kDa.
Conclusion:
The results suggested the hydrolysate and fractions under study contain bioactive peptides with potential for the development of antihypertensive drugs.
Related Concept Videos
Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors
Osmoregulation in Fishes
Antihypertensive Drugs: Vasodilators
Antihypertensive Drugs: Direct Renin Inhibitors
