[Diversity of HPA Antigen-antibody and Its Application in Platelet Transfusion in Shenzhen Area]

To investigate the polymorphism and genotype frequency of HPA gene in platelet donors in Shenzhen area, and to explore its application in clinical platelet transfusion.

The blood samples of 461 platelet donors aged 18-55 years old were genotyped for HPA-1~6,-10,-15 and -21 using multiple fluorescent PCR. Platelet-specific antibodies were detected according to the instructions of PAKPLUS kit.

The a and b alleles of HPA-3 and HPA-15 genes showed high frequency distribution, and their gene frequencies were HPA-3a (54.23%), HPA-3b (45.77%), HPA-15a(55.42%),HPA-15b(44.58%), respectively, and their dual antigen mismatch rate was the highest (37.32%, 37.20%). In HPA-21, the genotype distribution between male and female was statistically significantly different( P < 0.05). Among the patients with positive PAKPLUS test, 35.59% of patients were positive for platelet membrane glycoprotein antibody.

Some HPA antigens in Shenzhen area have high mismatch rates and significant regional differences. The investigation of HPA genes and antibodies diversity laid the foundation for establishing a local platelet donor and recipient HPA information database and addressing platelet alloimmunity issues.