Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Transplantation Into the Mouse Ovarian Fat Pad
09:25

Transplantation Into the Mouse Ovarian Fat Pad

Published on: September 7, 2016

TNFSF15 Promotes Vascular Normalization and Tertiary Lymphoid Structure Formation in Experimental Ovarian Cancer.

Jing-Ying Wang1, Yu-Ying Wang1, Li-Song Zhang1

  • 1State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, and Haihe Laboratory of Cell Ecosystem, Tianjin, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|June 2, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Fuzzy-Gaussian Modeling (DynFGM): A Kurtosis-Adaptive Unsupervised Framework for Automated Adipose Tissue Segmentation in Abdominal MRI.

Journal of imaging informatics in medicine·2026
Same author

A Microbial Lipid-ATP Synthase Axis Fuels NK Cell Antitumor Activity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A 4-guanidinobutanoic acid-SLC36A1 axis drives a microbiota‒host feedback loop to regulate intestinal homeostasis.

Gut microbes·2026
Same author

Activation of 5-HT<sub>1A</sub> receptors in the ventrolateral orbital cortex alleviates neuropathic pain-induced depressive-like behaviors via GABAergic neurons in male rats.

Neurochemistry international·2026
Same author

Netupitant/palonosetron is effective for preventing chemotherapy-induced nausea and vomiting in high-dose melphalan conditioning before autologous stem cell transplantation: A real-world analysis.

Blood science (Baltimore, Md.)·2026
Same author

Exploring different modification methods of biochar for Se(IV) removal from water: Synergistic effect of adsorption and reduction.

Journal of contaminant hydrology·2026

Tumor Necrosis Factor Superfamily-15 (TNFSF15) treatment inhibits ovarian cancer spread and ascites by normalizing vasculature and promoting immune cell infiltration. This enhances tumor responsiveness to immunotherapy.

Area of Science:

  • Oncology
  • Immunology
  • Cancer Biology

Background:

  • Ovarian cancer often shows reduced lymphocyte infiltration and poor response to immunotherapy.
  • Tumor microenvironment plays a critical role in cancer progression and treatment resistance.

Purpose of the Study:

  • To investigate the therapeutic potential of Tumor Necrosis Factor Superfamily-15 (TNFSF15) in a murine model of ovarian cancer.
  • To evaluate the effects of TNFSF15 on tumor vascularization, immune cell infiltration, and immunotherapy response.

Main Methods:

  • Treatment of ovarian cancer-bearing mice with recombinant TNFSF15.
  • Assessment of tumor dissemination, ascites, tumor vasculature (PDGFβ+ pericytes), hypoxia markers (CD133), lymphatic endothelial cells (Lyve-1+), high endothelial venules (PNAd+), and immune cell infiltration.
Keywords:
TNFSF15immunotherapyovarian cancertertiary lymphoid structurevascular normalization

More Related Videos

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery
07:40

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

Published on: May 16, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

Transplantation Into the Mouse Ovarian Fat Pad
09:25

Transplantation Into the Mouse Ovarian Fat Pad

Published on: September 7, 2016

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery
07:40

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

Published on: May 16, 2018

  • Analysis of tertiary lymphoid structures (TLS) formation and expression of TLS-associated cytokines/chemokines.
  • Evaluation of tumor response to PD-1 blockade post-TNFSF15 treatment.

    • Main Results:

    • TNFSF15 treatment significantly inhibited peritoneal cancer spread and reduced ascites.
    • Vascular normalization was observed, with increased pericyte coverage and decreased CD133 levels.
    • Accumulation of lymphatic endothelial cells and high endothelial venules indicated TLS formation.
    • TNFSF15 treatment facilitated infiltration of T cells, B cells, macrophages, and dendritic cells, with upregulated TLS-associated factors.
    • Enhanced responsiveness to PD-1 blockade was noted in TNFSF15-treated tumors.

    Conclusions:

    • TNFSF15 promotes vascular normalization and tertiary lymphoid structures (TLS) formation in ovarian cancer.
    • TNFSF15 treatment can restore a favorable immune microenvironment, enhancing anti-tumor immunity.
    • TNFSF15 represents a potential therapeutic strategy to improve ovarian cancer treatment outcomes, particularly in combination with immunotherapy.