Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Adhesion01:14

Adhesion

Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow glass...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum for Wiggins et al., "Inactivating conditions of therapeutic mycobacteriophages".

Microbiology spectrum·2026
Same author

Informing Equity-Oriented Approaches to Postpartum Care Following Severe Maternal Morbidity Events: A Qualitative Descriptive Study Protocol.

Global qualitative nursing research·2026
Same author

Inactivating conditions of therapeutic mycobacteriophages.

Microbiology spectrum·2026
Same author

Co-developing an inclusive interprofessional health workforce minimum data standard for enhanced planning and decision-making: A Canadian case with international relevance.

Health policy (Amsterdam, Netherlands)·2025
Same author

Implementation and Effectiveness of the Power Over Pain Portal for Patients Awaiting a Tertiary Care Consultation for Chronic Pain: Protocol for a Pilot, Prospective, Cohort, Mixed Methods Study.

JMIR research protocols·2025
Same author

Opioid analgesics for chronic noncancer pain in patients prescribed opioid agonist therapy or with opioid use disorder: A systematic review.

Canadian journal of pain = Revue canadienne de la douleur·2025

Related Experiment Video

Updated: Jun 3, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Molecular adhesion assay for biopolymer systems.

Jeremy A Cribb1, Farnaz Fazelpour2, David A Wollensak1

  • 1Department of Physics and Astronomy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

The Review of Scientific Instruments
|June 2, 2026
PubMed
Summary

We developed a micromagnetic bead assay to measure molecular adhesion forces in biological settings. This tool quantifies detachment forces, aiding research into mucus-pathogen interactions and drug delivery systems.

More Related Videos

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Related Experiment Videos

Last Updated: Jun 3, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Area of Science:

  • Biophysics
  • Materials Science
  • Biotechnology

Background:

  • Molecular adhesion is crucial in biology, but quantifying it in realistic environments is challenging.
  • Existing methods lack the resolution or applicability for physiologically relevant conditions.

Purpose of the Study:

  • To develop a scalable micromagnetic bead-based assay for quantifying molecular adhesion forces.
  • To measure detachment forces under physiologically meaningful conditions, particularly for mucoadhesion.

Main Methods:

  • Utilized a micromagnetic bead assay with controlled magnetic forces and high-speed microscopy.
  • Employed calibrated z-displacement mapping and in situ force calibration via Stokes drag.
  • Functionalized substrates and beads, validating with fluorescence imaging and zeta potential measurements.

Main Results:

  • Successfully quantified detachment forces in the sub-nanonewton to nanonewton range.
  • Demonstrated the assay's capability with various bead-substrate pairings, including mucus interactions.
  • Estimated binding energy and inferred dissociation constants from measured forces.

Conclusions:

  • The developed assay provides a scalable method for characterizing force-sensitive adhesive interactions.
  • Offers a novel approach for studying bioadhesive systems, including mucus-pathogen dynamics and drug delivery materials.
  • Enables bridging single-molecule resolution with biologically relevant adhesion quantification.