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Breaking the Cryogenic Sensing Limitation: Solvent-Microenvironment-Programmed Cold-Adaptable Nanozymes Enable

Xinyu Chen¹, Ruihan Zou¹, Jinjin Liu¹

¹School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China.

Analytical Chemistry

|June 2, 2026

Summary

Researchers developed a cold-adaptable nanozyme for detecting volatile amines (VAs) in cold food chains. This manganese-coordinated polyphosphate (Mn(DTPMP)) shows robust performance at low temperatures, overcoming limitations in cryogenic sensing.

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Area of Science:

Biochemical analysis
Materials science
Cryogenic sensing

Background:

Nanozymes offer advantages over natural enzymes but struggle with low-temperature efficiency.
Practical applications in cryogenic environments are limited by poor catalytic kinetics at low temperatures.

Purpose of the Study:

To develop a cold-adaptable nanozyme for sensitive detection of volatile amines (VAs) in food cold-chain logistics.
To overcome the limitations of low-temperature sensing in nanozymes.

Main Methods:

Synthesized solvent-microenvironment-programmed manganese-coordinated polyphosphate (Mn(DTPMP)) using hydrothermal methods with varying solvents.
Investigated the nanozyme's structure, surface chemistry, and catalytic properties across a wide temperature range.
Developed a colorimetric approach for VA detection leveraging the cold-adaptable nanozyme.

Main Results:

Mn(DTPMP) demonstrated robust oxidase-mimetic activity from near-zero to physiological temperatures.
The nanozyme's cold-adaptable feature is attributed to its composition, valence state, low activation energy, and high-spin electron configuration.
A sensitive colorimetric method for VA detection achieved a detection limit of 0.17 ppm in low-temperature environments.

Conclusions:

The developed Mn(DTPMP) nanozyme breaks the temperature barrier for sensitive cryogenic measurements.
This work provides insights into solvent-microenvironment regulation of nanozymes and their cold-adaptable mechanisms.
The findings inspire the design of advanced enzyme mimics for broader cryogenic applications.