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Updated: Jun 3, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Light-Dependent Regulation of Flavonol Biosynthesis through the HY5-MYB-FLS Module in Walnut
Na Wang1,2, Yi Jiang1,2, Erjuan Chen1,2
1College of Agriculture, Guizhou Engineering Research Center for Fruit Crops, Guizhou University, Guiyang 550025, China.
Abstract:
Light is a key environmental regulator of flavonol biosynthesis in plants. Previous studies identified JsMYB141, an R2R3-MYB transcription factor involved in this process in Juglans sigillata. However, the transcriptional mechanism by which light signals modulate flavonol biosynthesis remains unclear. To investigate this, we first showed that high light induces JsMYB141 and its target JsFLS4. Through homologous and heterologous functional validation, we confirmed that JsMYB141 and JsFLS4 significantly promote flavonol biosynthesis under high light. Additionally, overexpression of JsHY5 resulted in more pronounced upregulation of JsMYB141 and enhanced flavonol biosynthesis. Molecular interaction experiments demonstrated that both JsHY5 and JsMYB141 can activate the transcription of JsFLS4, either individually or cooperatively. These findings indicate that the JsHY5-JsMYB141-JsFLS4 module plays an important role in linking light signaling to the flavonol biosynthesis pathway in J. sigillata. This study provides a foundational theoretical framework for understanding the environmental regulation of secondary metabolism in woody plants.
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