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Protocol for quantifying and modeling light scattering in coral microskeletons
Netanel Kramer1, Steven L Jacques2, Daniel Wangpraseurt1
1Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA.
Abstract:
Light availability, which affects coral photosynthetic performance and health, is regulated by skeleton optical properties. Here, we present a non-invasive protocol for quantifying and modeling light scattering in coral microskeletons. We describe steps for extracting scattering coefficients and converting them to inherent optical properties using theoretical models. We then detail procedures for modeling Monte Carlo photon transport simulations on 3D coral geometries and calculating fluence distributions across skeleton and water layers. This approach enables high-resolution coral light-propagation characterization for ecological research. For complete details on the use and execution of this protocol, please refer to Kramer et al.1.
