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Updated: Jun 4, 2026
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
A novel molecularly imprinted electrochemical cellular sensor for DON detection
Donglei Jiang1, Yile Xu1, Nanwei Wang1
1College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, Jiangsu 210023, PR China.
Abstract:
This study developed a novel molecularly imprinted electrochemical cell sensor for ultrasensitive detection of deoxynivalenol (DON). Using o-phenylenediamine as the functional monomer and Caspase-3 as the template molecule, a molecularly imprinted polymer film was synthesized on screen-printed electrodes via electropolymerization. After elution, the sensor was integrated into a three-electrode system. Upon DON exposure, Caspase-3 released from HepG2 cells specifically binds to the imprinted cavities, altering the current response of the electrochemical indicator [Fe(CN)₆]4-/3-. Using differential pulse voltammetry (DPV), the peak current showed a linear relationship with DON concentration from 0.2 to 100 pg/mL, with a detection limit of 0.193 pg/mL (R2 = 0.9977). This work provides a new method for rapid DON detection in food and expands the application of molecular imprinting in mycotoxin analysis.
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