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Mechanisms underlying quality deterioration in pre-fermented frozen dough during freeze-thaw cycles: Insights from
Lu Lu1, Min Jiang1, Ayan Zong1
1School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, PR China; National Engineering Research Center for Deep Processing of Rice and By-products, Changsha 410004, Hunan Province, PR China.
Abstract:
This study investigated yeast-dough interaction by preparing dough with varying amounts of yeast and further elucidated the deterioration mechanism of pre-fermented frozen dough during repeated freeze-thaw (F/T) cycles. Results revealed that as the yeast inoculation (0%, 2% and 6%) in frozen dough increased, the specific volume of steamed bread decreased while its hardness increased. Specifically, yeast metabolism accelerated dough quality deterioration during F/T, as evidenced by reduced water retention and viscoelasticity, accelerated gluten depolymerization. Additionally, a non-targeted metabolomics analysis revealed that yeast metabolism significantly accumulated in yeast dough during F/T, such as fatty acids, carbohydrates, organic and amino acids. However, no such changes were observed in yeast-free dough. These experimental results demonstrated that the F/T process activated yeast metabolism, promoting metabolite accumulation and accelerating frozen dough quality deterioration. These results offer a new insight into the mechanisms underlying quality deterioration in frozen dough during F/T cycles.
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