Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthetic study of <i>vic</i>-bromination of diarylacetylenes, easy purification and separation.

Beilstein journal of organic chemistry·2026

Same author

Protein-based pan-RAS inhibitor induces tumor regression in female mice via IFNγ and CD8<sup>+</sup> T cell-dependent tumor necrosis.

Nature communications·2026

Same author

Development of a 3D-printed microfluidic chip for retinal organoid-endothelial co-culture.

Lab on a chip·2026

Same author

Metabolic positron emission tomography imaging and tumor growth inhibition during the Q neuron-induced hibernation-like state in mice.

Biochemical and biophysical research communications·2026

Same author

Perioperative Multidisciplinary Intervention Led to Complete Minimally Invasive Transthoracic Esophagectomy for a Patient With Severe Lung Dysfunction: A Case Report.

Cureus·2025

Same author

Beam commissioning of a new compact scanned proton therapy system with four different dose calculation algorithms.

Journal of applied clinical medical physics·2025

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

Microfluidic post-insertion preparation and evaluation of RGD- and PEG-modified macrophage-derived small

Mizuki Umino¹, Longjian Geng², Yusuke Ushiroda¹

¹School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo, Nagasaki-shi, Nagasaki, 852-8521, Japan.

Drug Metabolism and Pharmacokinetics

|June 2, 2026

Summary

A microfluidic method effectively modifies macrophage small extracellular vesicles (sEVs) with PEG and RGD lipids. This technique allows for simultaneous lipid incorporation, enhancing sEV targeting and functional applications.

Keywords:

Microfluidic post-insertion method Polyethylene glycol RGD Small extracellular vesicles Targeting

More Related Videos

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Jun 4, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Published on: June 30, 2023

Area of Science:

Biotechnology
Nanomedicine
Cell Biology

Background:

Small extracellular vesicles (sEVs) are crucial for intercellular communication.
Functionalization of sEVs enhances their targeted delivery capabilities.
Previous methods for sEV modification show source-dependent variations.

Purpose of the Study:

To evaluate the applicability of a microfluidic post-insertion method for modifying macrophage-derived sEVs.
To assess the simultaneous incorporation of polyethylene glycol (PEG) and arginine-glycine-aspartic acid (RGD) peptide lipids.
To compare microfluidic modification with conventional bulk mixing for sEV functionalization.

Main Methods:

Utilized a microfluidic post-insertion technique for sEV functionalization.
Incorporated PEG-lipids and RGD-peptide lipids into RAW264.7 macrophage-derived sEVs.
Compared physicochemical properties and cellular binding of modified sEVs using in vitro assays.

Main Results:

Microfluidic and bulk mixing methods yielded RGD-PEG sEVs with comparable properties and functions.
Successful co-incorporation of PEG and RGD lipids was confirmed.
PEGylation reduced overall sEV uptake, while RGD enhanced cellular association.

Conclusions:

The microfluidic method is effective for simultaneous PEG and RGD lipid incorporation into macrophage sEVs.
This approach offers a versatile strategy for functionalizing sEVs from diverse sources.
Co-functionalized sEVs demonstrate tailored cellular interaction profiles for potential therapeutic applications.