Iron redox-driven efficient nitrification-denitrification by Acinetobacter nosocomialis C24
Zongjian Huang1, Siqi Yang2, Liyi Pan1
1College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Abstract:
Simultaneous nitrification-denitrification and phosphate removal (SNDPR) are crucial for the development of single-reactor aerobic systems for wastewater treatment. This study demonstrated a significant enhancement of the SNDPR performance of Acinetobacter nosocomialis strain C24 following the addition of 1 mg/L Fe3+/Fe2+. Within 24 h, NH4+-N and PO43--P removal efficiencies increased by 33.1% and 26.9%, respectively. Under mixed nitrogen conditions, strain C24 achieved over 92% efficiency in removing total nitrogen (TN) and phosphate (PO43--P). The addition of Fe3+/Fe2+ also stimulated extracellular polymeric substance (EPS) secretion, with a notable positive correlation (p < 0.05) found between TN removal efficiency and levels of EPS, protein (PN), and polysaccharides (PS). Genomic analysis revealed that strain C24 utilizes dissimilatory nitrate reduction to ammonium (DNRA) for nitrogen metabolism. Transcriptomic analysis further indicated that Fe3+/Fe2+ intervention upregulated the expression of genes involved in iron redox, carbon metabolism, and nitrogen metabolism. Overall, these findings offer a thorough insight into the mechanisms by which Fe3+/Fe2+ enhanced nitrogen and phosphorus metabolism in strain C24. Given its excellent adaptability, high performance, and potential for widespread application, A. nosocomialis C24 emerges as a highly prospective option for wastewater treatment.
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