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Updated: Jun 4, 2026
Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle
Published on: September 22, 2015
Tunable peroxyl radical scavenging by graphene oxide dispersions: a multiparametric chemiluminescence metric
Anastasia A Denisova1, Iuliia A Poimenova2, Grigorii R Chermashentsev2
1Chemistry Department Physical Chemistry Division of Lomonosov Moscow State University, Moscow, 119234, Russia.
Background:
In biomedicine, a promising direction is the use of graphene oxide (GO) as a platform material or as a nanozyme. A key challenge for the application of GO is precise control of its interaction with reactive oxygen species, since this activity may influence the overall therapeutic outcome. Insignificant variations in material origin and processing can translate into measurable differences in its redox behavior. Peroxyl radical (ROO•)-generating assays are of particular interest, as ROO• are major contributors to oxidative stress in vivo, while kinetic chemiluminescence readouts sensitively track antioxidant effects of complex probes. This study addresses the need for a more biologically informed and comparable assessment of aqueous GO dispersions in relation to ROO•.
Results:
A chemiluminescence (CL) assay based on the well-known AAPH/luminol free radical generation system is presented. Buffer oxygenation is identified as a critical controllable factor: under otherwise identical conditions, oxygenation increased the analytical signal and stabilized the kinetic readout. Platinum-assisted conditioning is introduced as a diagnostic step to suppress peroxide-driven CL enhancement and reveal intrinsic GO behavior. A phenotype-guided strategy is developed and applied. Reproducibly Trolox-like traces are quantified in restricted Trolox-equivalent terms, whereas slow or non-Trolox-like responses are compared using fixed-time reactivity descriptors, enabling comparative series across a diverse set of GO dispersions, including commercial and laboratory-prepared samples as well as fractionated and non-fractionated materials.
Significance:
The combined workflow (buffer saturation & artefact removal & phenotype-guided quantification) improves robustness and interpretability of CL-based peroxyl radical assays for GO dispersions. It enables analytically justified comparison of GO samples differing in origin and processing history, while restricting Trolox-equivalent reporting to kinetically justified cases.
