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Updated: Jun 4, 2026
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
Published on: October 21, 2009
Electrospinning-constructed polysaccharide-based multilayer nanofiber composite membrane for bridging hemostasis and
Ruiyi Tan1, Zhou Wen1, Xinying He1
1State Key Laboratory of Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, 400715, China; Yibin Academy of Southwest University, Yibin, 644000, China.
Abstract:
The persistent gap between hemostasis and tissue repair in wound care demands new biomaterials. To address this, we engineered an electrospun polysaccharide-based multilayer composite nanofiber membrane (MCNM) designed to transform early blood clots into bioactive, pro-regenerative interfaces. The MCNM integrates distinct carbohydrate polymer functionalities: a wound-contacting layer of pullulan/tannic acid/tranexamic acid nanofibers for hemostasis and fibrinolysis suppression; a polydopamine/CaCl2-modified cellulose acetate intermediate layer for platelet adhesion and fibrin nucleation; and a chitosan-composited cellulose spunlace backing for directional exudate management. When applied, this structure quickly concentrates blood to create a stable clot that acts as both hemostatic barrier and reservoir or regenerative factors. Proteomic analysis confirmed significant upregulation of extracellular matrix organization, focal adhesion, and actin cytoskeleton remodeling pathways within the clot microenvironment. In vivo, the MCNM accelerated wound closure, improved re-epithelialization and collagen deposition, and promoted anti-inflammatory macrophage polarization. This work establishes an example for leveraging polysaccharide-driven clot bioengineering to seamlessly bridge hemostasis and tissue regeneration in advanced wound care.
