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Updated: Jun 4, 2026
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Plasmonic and surface-enhanced Raman nanobiosensors for quantitative molecular detection
Yeongbeom Kim1,2, Jaewon Choi1,3, Subin Lee1,2
1Division of Chemical Engineering and Bioengineering, College of Art, Culture and Engineering, Kangwon National University, Chuncheon, Gangwon State, 24341, Republic of Korea.
Discover Nano
|June 2, 2026
Summary
Plasmonic surface-enhanced Raman scattering (SERS) nanobiosensors offer ultrasensitive molecular detection. This review covers SERS principles, engineering, quantification, applications, challenges, and future directions for advanced biosensing.
Area of Science:
- Nanotechnology and Spectroscopy
- Biomedical Engineering
- Materials Science
Background:
- Plasmonic nanobiosensors leverage nanoscale electromagnetic fields for highly sensitive detection.
- Surface-enhanced Raman scattering (SERS) enables multiplexed analysis of various molecules.
- Current SERS technology faces challenges in reproducibility and standardization.
Purpose of the Study:
- To systematically review the principles, design, and application of plasmonic SERS nanobiosensors.
- To critically examine quantitative analysis methods and identify key performance-dictating factors.
- To discuss current limitations and propose future research directions in SERS biosensing.
Main Methods:
- Review of fundamental plasmonic principles and nanostructure engineering.
- Analysis of surface chemical functionalization strategies for sensor performance.
- Examination of quantitative methodologies including internal standards, ratio-based analysis, and machine learning.
Main Results:
- SERS nanobiosensors demonstrate potential in detecting nucleic acids, proteins, pathogens, and environmental toxins.
- Key factors influencing sensor performance include plasmonic principles, nanostructure design, and surface chemistry.
- Advanced quantification methods are crucial for reliable SERS data interpretation.
Conclusions:
- Plasmonic SERS nanobiosensors are powerful tools for ultrasensitive molecular detection with diverse applications.
- Addressing challenges in reproducibility, scalability, and standardization is vital for clinical and field deployment.
- Future research should focus on single-molecule detection, in vivo applications, and sustainable materials.
