Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hand hygiene

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...

Cleaning, Sterilization, and Disinfection

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Antimicrobial efficacy of hypochlorite-based gels for disinfecting heat-polymerized and 3D printed denture bases.

The Journal of prosthetic dentistry·2026

Same author

Time-series trends and regional disparities in complete denture production in Brazil (2000-2023): an epidemiological analysis.

Journal of applied oral science : revista FOB·2026

Same author

Biological Effects of Bovine Lactoferrin on Keratinocyte Proliferation and Migration (Bovine Lactoferrin and Wound Healing Response).

Journal of medicinal food·2026

Same author

Animal models in gunshot injury studies: A scoping review of model selection and quality assessment in ballistic research.

Forensic science, medicine, and pathology·2026

Same author

Influence of S-PRG-based restorative and adhesive systems on biofilm formation and enamel demineralization in a simulated oral environment.

Clinical oral investigations·2026

Same author

Bacterial Nanocellulose Functionalized with Graphite and Niobium Pentoxide: Limited Antimicrobial Effects and Preserved Cytocompatibility.

Membranes·2026

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

Published on: December 26, 2016

Chlorhexidine-based protocols for complete denture disinfection: a systematic review and meta-analysis.

Artur Ferronato Soto¹, Maria Eduarda Broering da Silva¹, Henrique Souza Dos Santos¹

¹Department of Dentistry, Federal University of Santa Catarina (UFSC), Florianopolis, SC, Brazil.

|June 2, 2026

Summary

Chlorhexidine effectively disinfects complete dentures, significantly reducing microbial load regardless of concentration or application method. Further research is needed to standardize protocols for optimal dental practice.

Keywords:

Biofilms Chlorhexidine Colony Count Denture Bases Disinfection Microbial Therapeutic Uses

More Related Videos

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Published on: March 14, 2025

Related Experiment Videos

Last Updated: Jun 4, 2026

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

Published on: December 26, 2016

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Published on: March 14, 2025

Area of Science:

Dentistry
Microbiology
Infectious Diseases

Background:

Complete denture use is common, increasing the risk of microbial colonization and infections.
Effective disinfection protocols for complete dentures are crucial for oral health.
Chlorhexidine is a widely used antimicrobial agent with potential for denture disinfection.

Purpose of the Study:

To systematically review and evaluate the effectiveness of chlorhexidine-based protocols for disinfecting complete dentures.
To compare the antimicrobial efficacy of different chlorhexidine concentrations, vehicles, and application protocols.

Main Methods:

A comprehensive systematic literature search was conducted across multiple databases up to September 2024.
Included were clinical studies assessing microbial load reduction (CFU) on dentures after chlorhexidine use.
Meta-analysis and GRADE certainty assessment were employed to analyze antimicrobial efficacy.

Main Results:

Twenty-two studies were included, with five contributing to the meta-analysis.
Chlorhexidine demonstrated significant reduction in CFU counts across various concentrations (0.12% and 0.2%) and application methods (immersion, rinses, sprays).
Pooled analysis confirmed significant microbial reduction over time, indicating chlorhexidine's broad effectiveness.

Conclusions:

Chlorhexidine-based protocols are effective in disinfecting complete dentures, reducing microbial load.
Effectiveness is observed irrespective of chlorhexidine concentration or mode of application.
Standardized clinical studies are recommended to establish optimized and reproducible disinfection protocols for dental practice.