Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative effectiveness of dual antiplatelet therapy after revascularization in diabetes and chronic limb-threatening ischemia: a target trial emulation study.

Cardiovascular diabetology·2026
Same author

Mechanism-Based Inactivation of Human Ornithine Aminotransferase by Ethynyl- and Nitrile-Substituted Cyclopentene Analogues of γ-Aminobutyric Acids.

Journal of the American Chemical Society·2026
Same author

Deep Proteoform Sequencing with Top-Down Direct Mass Technology.

bioRxiv : the preprint server for biology·2026
Same author

CSF ctDNA analysis guides molecular reclassification of diffuse glioma patients.

Journal of neuro-oncology·2026
Same author

KLK10 Upregulation Drives Aggressiveness and Radioresistance and Has a Negative Prognostic Impact on Rectal Cancer.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

SMARCA2 PROTAC-dendrimer conjugates for the treatment of non-small cell lung cancer.

Journal of pharmaceutical sciences·2026

Related Experiment Video

Updated: Jun 4, 2026

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules
04:24

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules

Published on: June 16, 2023

Characterizing Antibody-Drug Conjugates with High Molecular Heterogeneity by Native Direct Mass Technology.

Phillip Y Chu1, Keely E Fuller1, Summer Baker Dockrey2

  • 1Department of Biochemical and Cellular Pharmacology, Genentech Inc., South San Francisco, California 94080, United States.

Analytical Chemistry
|June 3, 2026
PubMed
Summary

Direct mass technology (DMT) using Orbitrap MS offers sensitive, automated analysis of antibody-drug conjugate (ADC) drug-to-antibody ratio (DAR) and stability, even in complex biological samples.

More Related Videos

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
11:02

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules
04:24

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules

Published on: June 16, 2023

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
11:02

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Mass spectrometry (MS) is crucial for characterizing antibody-drug conjugates (ADCs).
  • Accurate determination of drug-to-antibody ratio (DAR) and ADC stability is essential.
  • Conventional native MS methods face limitations with heterogeneous ADCs.

Purpose of the Study:

  • To present a native direct mass technology (DMT) approach for ADC characterization.
  • To enable automated, highly sensitive analysis of DAR and stability for high DAR ADCs.
  • To evaluate the platform's performance across various sample matrices and concentrations.

Main Methods:

  • Utilized an Orbitrap-based charge detection MS integrated with SampleStream and Newomics' MnESI source.
  • Applied native direct mass technology (DMT) for analyzing interchain-modified high DAR ADCs (DAR 14).
  • Tested the platform with samples from formulation buffer, ex vivo, and in vivo conditions.

Main Results:

  • Achieved direct DAR characterization for heterogeneous DAR-14 ADCs, which is challenging for conventional native MS.
  • Demonstrated automated and highly sensitive analysis of ADC DAR and stability.
  • Successfully assessed interchain stability in biomatrices, even at low ADC concentrations.

Conclusions:

  • Native DMT provides a robust, high-resolution method for characterizing interchain-modified high DAR ADCs.
  • This approach overcomes limitations of conventional MS for heterogeneous ADC analysis.
  • Native DMT is effective across diverse biological environments and low sample concentrations.