Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbiota-derived indole derivatives as anticancer agents: mechanistic insights and major perspectives.

Future microbiology·2026
Same author

Integrated Genomic Profiling of Newly Diagnosed and Relapsed Acute Myeloid Leukemia Identifies Driver Genes, Mutational Signatures, and Therapeutic Targets.

Cancers·2026
Same author

pH-responsive dual-drug-loaded bovine serum albumin nanoparticles for targeted cancer therapy.

International journal of biological macromolecules·2026
Same author

A low-cost disposable label-free immunosensor for high-performance detection of indoxyl sulfate using chitosan based tertiary nanocomposite.

International journal of biological macromolecules·2026
Same author

Implantation of standard defibrillator leads in the left bundle branch area: Experience from an international cohort.

Heart rhythm·2026
Same author

DPYD genotyping in patients receiving capecitabine: an exploratory analysis from the D-TORCH study.

Frontiers in pharmacology·2026

Related Experiment Video

Updated: Jun 4, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

QuaDB: A streamlined web tool for identifier-based rapid prediction of putative quadruplex sequences.

Auroni Deep1, Utsab Das2, Perumal Vivekanandan3

  • 1Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

Human Genomics
|June 3, 2026
PubMed
Summary

QuaDB is a new web tool for rapid prediction of Putative Quadruplex Sequences (PQS) using gene identifiers. It streamlines analysis of G-quadruplexes and i-motifs, aiding large-scale genomic studies.

Keywords:
G-QuadruplexesPutative Quadruplex Sequences (PQS)Web applicationi-Motifs

More Related Videos

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Non-canonical DNA structures like G-Quadruplexes (G4s) and i-Motifs (iMs) are crucial for gene regulation.
  • Current methods for identifying their genomic precursors are manual and hinder large-scale analysis.
  • QuaDB addresses this limitation for efficient G4 and iM precursor identification.

Purpose of the Study:

  • To develop a user-friendly web application, QuaDB, for rapid, identifier-based prediction of Putative Quadruplex Sequences (PQS).
  • To streamline the analysis of G4s and iMs across different genomic regions.
  • To facilitate large-scale genomic investigations of non-canonical DNA structures.

Main Methods:

  • Real-time sequence retrieval from Ensembl and NCBI using gene identifiers.
  • Automated, region-specific analysis (promoter, gene body, 3' downstream).
  • A biologically informed scoring model for accurate quadruplex structure identification and scoring.

Main Results:

  • QuaDB enables efficient, identifier-based retrieval and region-aware analysis of PQS.
  • The tool's scoring model accurately identifies quadruplex structures.
  • Experimental validation confirmed a correlation between QuaDB scores and thermal stability.

Conclusions:

  • QuaDB facilitates comprehensive, large-scale genomic investigations of G4s and iMs.
  • It complements existing tools with its unique identifier-based and region-aware pipeline.
  • The tool aids in exploring the biological significance of non-canonical DNA structures.