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Related Experiment Video

Updated: Jun 4, 2026

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
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Published on: March 24, 2023

Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.

Jiazhu Xu1, Joel Aboagye2, Marcella Edwards2

  • 1Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA.

Advanced Functional Materials
|June 3, 2026
PubMed
Summary
This summary is machine-generated.

We developed novel cardiac extracellular matrix (ECM) microspheres to mature human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). This 3D biomaterial platform significantly enhances hiPSC-CM maturation for cardiac research and tissue engineering.

Keywords:
Decellularized cardiac ECMMaturationMicrospherehiPSC-CMs

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Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Phenotypic immaturity of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hinders their use in disease modeling and cardiac regeneration.
  • Existing culture platforms struggle to provide a physiologically relevant microenvironment for cardiomyocyte maturation.

Purpose of the Study:

  • To develop a novel biomaterial platform for enhanced hiPSC-CM maturation.
  • To investigate the efficacy of cardiac extracellular matrix (ECM) microspheres in supporting hiPSC-CM development.

Main Methods:

  • Fabrication of reproducible, heart tissue-derived cardiac ECM microspheres with a porous 3D architecture.
  • Culture of various cell types, including hiPSC-CMs, on ECM microspheres.
  • Assessment of hiPSC-CM maturation through gene expression, calcium handling, sarcomere organization, and protein expression.

Main Results:

  • ECM microspheres supported attachment and proliferation of multiple cell types, including hiPSC-CMs.
  • Compared to 2D cultures, ECM microspheres significantly enhanced hiPSC-CM maturation, evidenced by upregulated cardiac genes (ACTA2, TNNT2, GJA1), improved calcium cycling, and synchronized transients.
  • Long-term culture (up to 8 months) on ECM microspheres promoted hiPSC-CM maturation, with enhanced sarcomere alignment, α-actinin expression, contractile function, connexin 43 (CX-43) expression, and binucleation.

Conclusions:

  • Cardiac ECM microspheres provide a scalable, bioactive 3D platform for long-term hiPSC-CM culture and maturation.
  • This system holds promise for advancing cardiac disease modeling, drug screening, and cardiac tissue engineering applications.