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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 4, 2026

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Published on: June 18, 2012

Flow cytometry method to identify arginase-1 expressing MDSCs.

Pugazhendhi Srinivasan¹, Misty Bechtel¹, Dorota Wyczechowska²

¹Department of Urology, University of Kansas Medical Center, Kansas City, Kansas, USA.

|June 3, 2026

Summary

This summary is machine-generated.

A new flow cytometry method quantifies intracellular arginase-1 (ARG1) in myeloid-derived suppressor cells (MDSCs). This technique accurately measures ARG1 in specific cell types, aiding immunosuppression research in cancer.

Keywords:

Cancer Flow cytometry Immunosuppression Intracellular staining MDSC

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Published on: June 9, 2021

Area of Science:

Immunology
Cancer Biology
Cellular Assays

Background:

Myeloid-derived suppressor cells (MDSCs) mediate immune suppression in cancer via arginase-1 (ARG1).
Existing assays like ELISA and qPCR cannot pinpoint the cellular origin of ARG1 in blood.
Accurate quantification of ARG1 expression in specific cell subsets is crucial for understanding MDSC function.

Purpose of the Study:

To develop and validate a standardized flow cytometry method for measuring intracellular ARG1 in MDSC subsets.
To provide a reliable protocol for researchers to quantify ARG1 expression in specific cell populations.
To facilitate studies on MDSC-mediated immunosuppression.

Main Methods:

Developed a flow cytometry protocol using a commercial ARG1 antibody for intracellular staining.
Validated antibody specificity with positive (neutrophils) and negative (CD4+ T cells) controls.
Applied the method to peripheral blood mononuclear cells from bladder cancer patients and healthy volunteers.

Main Results:

Successfully established and validated a flow cytometry method for intracellular ARG1 detection.
Demonstrated reliable quantification of ARG1 in MDSC subpopulations from patient and healthy samples.
Verified antibody specificity, ensuring accurate measurement of ARG1 expression.

Conclusions:

The developed flow cytometry method offers a standardized approach to measure cell-specific ARG1 expression.
This technique is adaptable to various cell types and valuable for immunosuppression research.
Provides a tool to better understand the role of ARG1 in MDSC-mediated immune suppression.