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Updated: Jun 4, 2026
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Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
Published on: July 27, 2022
Lacto-N-biose Improves Acute Colitis in Mice by Modulating Gut Microbiota and Metabolites.
Zixin Fan1, Wanying Xie1, Xiaopei Hu1
1College of Food Science, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang 110866, China.
Journal of Agricultural and Food Chemistry
|June 3, 2026
Summary
Lacto-N-biose (LNB) effectively treats colitis in mice by reducing inflammation and improving gut barrier function. This human milk oligosaccharide also rebalances gut microbiota and may influence bile acid pathways.
Area of Science:
- Gastroenterology
- Immunology
- Microbiology
Background:
- Human milk oligosaccharides (HMOs) are crucial for infant health.
- Lacto-N-biose (LNB), a core HMO structure, lacks sufficient characterization regarding its anti-colitis potential.
Purpose of the Study:
- To investigate the therapeutic effects of LNB on dextran sulfate sodium salt-induced colitis in a mouse model.
- To elucidate the mechanisms underlying LNB's efficacy in ameliorating colitis.
Main Methods:
- Induction of colitis in mice using dextran sulfate sodium salt.
- Administration of LNB at varying doses.
- Assessment of disease activity index, immune factor levels (TNF-α, F4/80, IL-6, IFN-γ, IL-4), colonic architecture, and intestinal barrier markers (Muc2, ZO-1, Occludin, Claudin1).
- Analysis of gut microbial composition and fecal metabolomics.
Main Results:
- LNB significantly ameliorated colitis symptoms and reduced inflammatory markers.
- LNB protected colonic architecture and enhanced intestinal barrier integrity.
- LNB restored gut microbial balance, promoting beneficial bacteria like Akkermansia and Lachnospiraceae bacterium UBA3282.
- LNB modulated fecal metabolites, including downregulation of 7α-hydroxycholesterol, suggesting involvement of bile acid pathways.
Conclusions:
- LNB demonstrates significant therapeutic potential for colitis.
- LNB's efficacy involves immune modulation, gut barrier repair, and microbiota restoration.
- LNB may exert its beneficial effects through pathways related to bile secretion and primary bile acid biosynthesis.
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