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Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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Related Experiment Video

Updated: Jun 4, 2026

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
10:24

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

Intrinsically disordered regions facilitate Mlp1-Nab2 recognition in mRNA quality control.

Mohammad Soheilypour1, Mohaddeseh Peyro1, Hengameh Shams1

  • 1Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California, Berkeley, CA, USA.

Nucleus (Austin, Tex.)
|June 3, 2026
PubMed
Summary

Messenger RNA (mRNA) quality control relies on proteins like Myosin-like protein 1 (Mlp1) interacting with RNA-binding proteins (RBPs). This study reveals Mlp1-Nab2 binding involves Nab2

Keywords:
RNA-binding proteins (RBPs)intrinsically disordered proteinsmRNA exportmRNA quality controlmolecular dynamics simulationsnuclear basketnuclear pore complex (NPC)

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Last Updated: Jun 4, 2026

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
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Published on: December 17, 2012

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Nuclear mRNA quality control ensures proper transcript export.
  • Myosin-like protein 1 (Mlp1) interacts with RNA-binding proteins (RBPs) like Nab2.
  • The role of Phe73 in Nab2 for Mlp1 binding is not fully understood.

Purpose of the Study:

  • To elucidate the molecular mechanism of Mlp1-Nab2 interaction.
  • To develop a mechanistic model for Mlp1-Nab2 binding using computational approaches.

Main Methods:

  • Computational modeling to analyze Mlp1-Nab2 interactions.
  • Investigating the impact of Nab2 Phe73 mutations (F73A, F73W) on binding.

Main Results:

  • Nab2's Phe73 stabilizes intramolecular helix interactions, promoting a compact conformation, rather than direct Mlp1 contact.
  • Mutation F73A weakened Mlp1-Nab2 binding by disrupting helix stabilization.
  • Mutation F73W enhanced Mlp1-Nab2 interaction.

Conclusions:

  • Mlp1-Nab2 binding is mediated by Nab2's structural flexibility and Mlp1's disordered domain.
  • This adaptive recognition mechanism is crucial for mRNA quality control by the nuclear basket.
  • Flexible protein-protein recognition is vital for nuclear mRNA surveillance.