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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Jun 4, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

In Situ Amplified Mutational mRNA Imaging Using a Spatially Confined CRISPR Nanoplatform.

Wen Zhao1, Ziyue Zheng1, Rui Li1

  • 1School of Pharmaceutical Sciences, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, China Pingyuan Laboratory, Tianjian Laboratory of Advanced Biomedical Sciences, State Key Laboratory of Antiviral Drugs, Zhengzhou University, Zhengzhou, Henan, China.

Angewandte Chemie (International Ed. in English)
|June 3, 2026
PubMed
Summary

We developed a CRISPR/Cas13a-based nanoprobe system (InCasRD) for rapid, sensitive spatial RNA detection and mutation mapping in tissues. This tool aids in understanding cellular heterogeneity and disease, with potential for precision medicine.

Keywords:
CRISPR/Cas13aRNA detectionin situ imagingsingle nucleotide variantstissue sections

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Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Accurate spatial analysis of RNA mutations is crucial for understanding cellular heterogeneity and disease.
  • Existing methods for RNA detection in tissues can be limited in sensitivity and speed.

Purpose of the Study:

  • To develop an integrated CRISPR/Cas13a-based nanoprobe system for rapid and sensitive detection of RNA in tissue sections.
  • To enable spatial mapping of RNA mutations for improved tumor delineation and diagnosis.

Main Methods:

  • Development of the Integrated CRISPR/Cas13a-based RNA Rapid Detection (InCasRD) system.
  • Utilizing the trans-cleavage activity of Cas13a for spatially confined signal amplification and high signal-to-background ratio.
  • Imaging of multiple RNA targets (mRNA, microRNA, circular RNA) and RNA mutations (EGFR L858R, OTUD SNV) in tumor tissue sections.

Main Results:

  • Achieved rapid imaging of multiple target RNAs in tumor cells within 0.5 hours of incubation.
  • Successfully mapped RNA mutations, including specific EGFR and OTUD variants, in tumor tissue sections.
  • Demonstrated high signal-to-background ratio for spatially confined RNA detection.

Conclusions:

  • InCasRD is a powerful, one-step tool for in situ RNA analysis.
  • The system enables sensitive detection and spatial mapping of RNA mutations.
  • InCasRD holds significant potential for diagnosis and precision medicine applications.