Environmental fungi modulate the vaginal mycobiome and cervical disease progression in Hispanic women

The vaginal mycobiome, though a minor component of the cervicovaginal ecosystem, plays a crucial role in reproductive health and disease. However, its composition and interactions with bacterial communities remain poorly understood, particularly among Hispanic women, who experience disproportionately high rates of human papillomavirus (HPV) infection and cervical cancer. We characterized the vaginal mycobiota across reproductive stages and examined its associations with cervical disease, HPV status, and bacterial community state types (CSTs) in 86 Hispanic participants from Puerto Rico using ITS1 amplicon sequencing. Amplicon sequence variants were inferred with QIIME2/DADA2 and taxonomically classified using the UNITE database, with diversity and discriminant taxa analyses applied to explore clinical and microbial associations. We detected 173 fungal species hypotheses, dominated by Candida albicans, Agaricomycetes sp., Scopuloides dimorpha, and Hortaea werneckii. While fungal composition did not differ significantly by reproductive stage, non-pregnant individuals exhibited greater inter-individual variability. Alpha diversity was reduced in high-grade squamous intraepithelial lesions compared with low-grade or normal cytology, and Candida parapsilosis prevalence was elevated in low-grade lesions. CST III, characterized by Lactobacillus iners dominance, showed greater dispersion variance than other CSTs. Collectively, these findings reveal a diverse vaginal mycobiome with stage- and disease-specific features and a notable contribution of environmental fungi that may influence cervical pathogenesis. This work provides foundational insight into cervicovaginal fungal ecology in a high-risk Hispanic population and highlights the importance of integrating bacteriome-mycobiome analyses in women's health research.IMPORTANCEThis study demonstrates that environmental fungi may play an important role in shaping the vaginal microbiome and influencing cervical disease progression in Hispanic women. While most prior research has focused on bacterial communities, our findings highlight fungi as significant yet underexplored contributors to reproductive health. By linking environmental fungal exposures to shifts in the vaginal mycobiome, this work expands understanding of how external factors may affect disease risk. These results underscore the need to include fungal communities in human microbiome research and support more comprehensive approaches to women's health. Ultimately, this study may inform improved risk assessment, prevention strategies, and the development of targeted interventions in diverse populations.