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Related Experiment Video

Updated: Jun 4, 2026

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
09:07

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Published on: May 24, 2024

RAST+: pre-positivity application of EUCAST RAST using BACTEC FX growth detection plots - a proof-of-concept study.

Kutay Demirel1, Ali Fazıl Anıl2, Neşe İnal Kibar3

  • 1Faculty of Health Sciences, Balıkesir University, Balıkesir, Türkiye.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|June 3, 2026
PubMed
Summary

This study introduces a novel rapid antimicrobial susceptibility testing (RAST+) method that analyzes Growth and Detection Plots (GDPs) before blood culture positivity. This approach accelerates antimicrobial susceptibility testing results, aiding faster clinical decisions.

Keywords:
Growth and detection plotsRASTRAST+

Related Experiment Videos

Last Updated: Jun 4, 2026

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
09:07

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Published on: May 24, 2024

Area of Science:

  • Clinical microbiology
  • Diagnostic development
  • Antimicrobial stewardship

Background:

  • Timely antimicrobial susceptibility testing (AST) from blood cultures is critical for effective antimicrobial therapy.
  • Current methods can have significant turnaround times, delaying optimal treatment.
  • Accelerating AST is essential to combat antimicrobial resistance and improve patient outcomes.

Purpose of the Study:

  • To accelerate AST results by applying the EUCAST RAST method before blood culture positivity (RAST+).
  • To utilize Growth and Detection Plots (GDPs) from the BD BACTEC FX system for early detection of bacterial growth.
  • To evaluate the feasibility and accuracy of performing RAST+ directly from blood culture bottles.

Main Methods:

  • Inoculated quality control strains and clinical isolates into aerobic blood culture bottles.
  • Monitored Growth and Detection Plots (GDPs) for early bacterial growth detection.
  • Performed rapid antimicrobial susceptibility testing (RAST+) using a 300 µL inoculum prior to signal positivity.

Main Results:

  • The RAST+ method demonstrated interpretable inhibition zones with a 300 µL inoculum for 85.9% of tested antibiotic disks.
  • RAST+ achieved a high categorical agreement (96.2%) compared to the reference method.
  • Reported very major, major, and minor error rates of 1.3%, indicating high diagnostic reliability.

Conclusions:

  • This study is the first to use GDPs to predict blood culture positivity and apply RAST before signal detection.
  • RAST+ is a proof-of-concept for performing AST directly from blood cultures pre-positivity.
  • This method has the potential to significantly reduce turnaround times and support earlier clinical decision-making.