Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the Performance of Mass Spectrometry Search Strategies in Identifying Translational Errors Using PDX Proteomics Data.

Molecular & cellular proteomics : MCP·2025
Same author

Assessment of radioactivity in soil from the vicinity of Srikail gas field, Bangladesh: implications for public health and environment.

Isotopes in environmental and health studies·2025
Same author

Evaluating and Optimizing Mass Spectrometry Proteomics Data to Deconvolve Cell-Type-Specific Protein Expression in Tumors.

Journal of proteome research·2025
Same author

Natural radioactivity in soil and concomitant hazards in the BSCIC Industrial Area, Jhenaidah, Bangladesh: a pioneering study.

Isotopes in environmental and health studies·2025
Same author

Radiological assessment and potential health risks of naturally occurring radioactive materials in Kuakata Sea Beach sand: implications for rare-earth resources and nuclear fuel cycle feasibility.

Isotopes in environmental and health studies·2025
Same author

Radiometric study and associated radiological hazards in soil near the Bheramara 410 MW Combined Cycle Power Plant, Kushtia, Bangladesh.

Isotopes in environmental and health studies·2025

Related Experiment Video

Updated: Jun 5, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

IsoPepTracker: An interactive web application for peptide-driven isoform analysis.

Araf Mahmud1, Chen Huang1,2

  • 1Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.

Plos Computational Biology
|June 3, 2026
PubMed
Summary
This summary is machine-generated.

IsoPepTracker analyzes protein isoforms from alternative splicing events, bridging RNA and protein insights. This tool helps identify differential peptides for shotgun proteomics validation.

More Related Videos

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Related Experiment Videos

Last Updated: Jun 5, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Area of Science:

  • Proteogenomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing impacts 95% of multi-exon genes, producing diverse protein isoforms.
  • Current RNA-level analyses offer limited insight into protein-level changes.
  • A gap exists in analyzing protein consequences of alternative splicing.

Purpose of the Study:

  • To introduce IsoPepTracker, a web application for analyzing differential peptides across protein isoforms.
  • To provide protein-level insights into alternative splicing events.
  • To facilitate proteogenomics research by linking peptides to genes and isoforms.

Main Methods:

  • Development of IsoPepTracker, a user-friendly web application with four modules: Canonical Isoform Analysis, Novel Isoform Discovery, Peptide Sequence Search, and Alternative Splicing Analysis.
  • Integration of capabilities to analyze canonical and novel isoforms.
  • Enabling analysis of peptides theoretically detectable by shotgun mass spectrometry.

Main Results:

  • IsoPepTracker enables analysis and visualization of differential peptides related to alternative splicing.
  • The application supports input of genes, novel cDNA sequences, peptides, or alternative splicing results.
  • Case studies demonstrate the straightforward application of IsoPepTracker in proteogenomics.

Conclusions:

  • IsoPepTracker provides informative peptide signatures to understand protein-level consequences of alternative splicing.
  • The tool supplies peptide candidates for validation in shotgun proteomics experiments.
  • IsoPepTracker bridges the gap between RNA-level splicing events and protein-level isoform diversity.