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Related Experiment Video

Updated: Jun 5, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

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Published on: October 14, 2022

Rice ONAC005 inhibits precocious leaf senescence through a feed-forward regulatory loop involving OsNAP.

Jinku Kang¹, Sang-Il Bae¹, Eunji Shin¹

¹Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.

Plant Physiology

|June 3, 2026

Summary

ONAC005 acts as a leaf senescence repressor. Its decline triggers leaf yellowing, while its overexpression delays senescence by downregulating key genes and pathways, including OsNAP.

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Area of Science:

Plant Molecular Biology
Plant Physiology
Gene Regulation

Background:

Leaf senescence is a crucial developmental stage regulated by complex genetic networks.
Understanding the molecular mechanisms controlling leaf senescence is vital for crop longevity and yield.

Purpose of the Study:

To investigate the role of ONAC005 in regulating leaf senescence.
To elucidate the molecular mechanism by which ONAC005 controls senescence.

Main Methods:

Gene expression analysis (natural and dark-induced senescence).
Mutagenesis (T-DNA insertion and CRISPR/Cas9) and overexpression studies in plants.
Abscisic acid (ABA) response assays.
In vivo and in vitro DNA-binding assays to identify direct targets.

Main Results:

ONAC005 expression decreases during leaf senescence.
onac005 mutants show premature senescence, while ONAC005 overexpression delays it.
ONAC005 represses senescence-associated genes (SAGs) and chlorophyll degradation genes (CDGs).
ONAC005 directly binds to OsNAP and its regulated gene promoters, inhibiting transcription.
ONAC005 dampens the abscisic acid (ABA)-induced senescence response.

Conclusions:

ONAC005 functions as a negative regulator of leaf senescence.
It acts by repressing the OsNAP-mediated senescence signaling pathway.
ONAC005 is a key target for manipulating leaf senescence in plants.