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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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Intelligent fluorophores: navigating biological complexity through adaptive single-molecule imaging

Byung Seok Cha1, Hyo In Kim2, Taekjip Ha3

  • 1College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.

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