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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
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A smartphone-powered decentralized microfluidic platform for sensitive CRISPR-based nucleic acid detection.

Peng Zhou1, Amber N McElroy1, Yingming Xu1

  • 1University of Minnesota, Minneapolis, MN, USA.

Microsystems & Nanoengineering
|June 3, 2026
PubMed
Summary
This summary is machine-generated.

This study presents a low-cost, portable nucleic acid detection device using laser-induced graphene (LIG) heaters for loop-mediated isothermal amplification (LAMP) and CRISPR/Cas9. The platform achieves sensitive DNA/RNA detection, ideal for resource-limited settings.

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Materials Science

Background:

  • Point-of-care diagnostics require sensitive, portable, and low-cost nucleic acid detection methods.
  • Resource-limited settings face challenges with complex equipment and trained personnel for molecular diagnostics.

Purpose of the Study:

  • To develop an integrated, low-power diagnostic platform for nucleic acid amplification and detection.
  • To enable sensitive and flexible molecular diagnostics adaptable for field use.

Main Methods:

  • Development of a microfluidic chip with a laser-induced graphene (LIG) heater for precise temperature control during loop-mediated isothermal amplification (LAMP).
  • Integration of CRISPR/Cas9 for molecular interrogation of amplified DNA/RNA.
  • Detection using lateral flow assays (LFAs) and an electrochemical module with a RuHex redox probe.
  • An Arduino-based control unit powered by smartphone energy was developed.

Main Results:

  • Achieved DNA/RNA amplification and detection limits as low as 5 copies per 10 μL using LIG-based LAMP.
  • Demonstrated detection limits of 10^11 copies/μL for LFA and 5 × 10^6 copies/μL for the electrochemical platform.
  • The integrated system operated on smartphone power with low steady-state power consumption (~130 mW for LIG heater).

Conclusions:

  • The developed LIG-based microfluidic device offers a low-cost, low-power solution for sensitive nucleic acid detection.
  • This platform has significant potential for point-of-care applications, particularly in resource-limited environments.
  • The system integrates amplification and detection, enabling flexible forward diagnostics.