Related Experiment Video
Updated: Jun 5, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
Pan-genome based on chromosome sequences of wild and cultivated Agaricus bisporus
Yanquan Qi1,2,3,4, Siyang Guo2,3, Zhenhua Liu1,3
1International Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agriculture University, Changchun, Jilin, China.
Abstract:
Agaricus bisporus, one of the most widely cultivated mushrooms around the world, plays an important role in economy and agriculture. In this study, by employing long-reads generated by PacBio and Nanopore sequencing, we assembled six novel high-quality genomes (of which three are telomere-to-telomere assemblies) with sizes 29.6 ~ 30.8 Mb and N50 lengths of 2.5 ~ 2.6 Mb. Combined with public genome data of nine strains, we successfully established a pan-genome of A. bisporus, comprising a total of 14,626 clusters of protein coding genes, of which 50.70%, 7.45%, 24.74% and 17.01% are defined as core, soft core, dispensable, and private clusters, respectively. A total of 5,646 non- redundant structural variants (SVs) were identified among wild and cultivated strains and the genes associated with SV were mapped. This work provides valuable whole-genome sequences and genomic resources across wild and cultivated strains of the most widely cultivated mushroom species for functional analyses of genomes.
Related Concept Videos
Fungal Phylum Basidiomycota
Evolution of Microbial Genome
Modern Molecular Taxonomy
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Phylogenetic Species Concept in Microbiology

