Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Microbial Corrosion01:24

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
Biodeterioration01:28

Biodeterioration

Biodeterioration refers to the unwanted alteration of materials caused by microorganisms—especially fungi—which damage both organic substrates (paper, wood, textiles) and inorganic ones (stone, plaster, glass). Unlike abiotic decay, biodeterioration results from biological activity that produces physical disruption and chemical degradation.Physical deterioration occurs as fungal hyphae penetrate pores, cracks, and surface irregularities. Hyphal turgor pressure, thigmotropic growth along...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.

Nature·2026
Same author

Dynamic conformational ensembles of soluble Tau encode neuronal toxicity prior to aggregation.

bioRxiv : the preprint server for biology·2026
Same author

Chewing Affects Structural and Material Coupling, and Age-Related Dentoalveolar Joint Biomechanics and Strain.

Bioengineering (Basel, Switzerland)·2026
Same author

Detecting patterns of atrophy in cognitively impaired individuals using portable, low-field MRI.

Imaging neuroscience (Cambridge, Mass.)·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Clinical Manifestations.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Related Experiment Video

Updated: Jun 5, 2026

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

Silver diamine fluoride alters microbial communities in subsurface dentin.

Rosalyn M Sulyanto1,2, Clifford J Beall3, Martin T Berger1,2

  • 1Department of Dentistry, Boston Children's Hospital, Boston, MA.

JADA Foundational Science
|June 4, 2026
PubMed
Summary

Silver diamine fluoride (SDF) does not alter surface biofilm but shifts microbial communities within dentin tubules, revealing a new mechanism for caries arrest. This finding impacts understanding of SDF’s effectiveness in pediatric dentistry.

Keywords:
16S rRNA gene sequencingBiomaterialbiofilmbioinformaticscaries arrestmicrobiotapediatric dentistry

More Related Videos

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Related Experiment Videos

Last Updated: Jun 5, 2026

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Area of Science:

  • Dentistry
  • Microbiology
  • Biomaterials

Background:

  • Silver diamine fluoride (SDF) is a dental biomaterial used for its caries-arresting properties.
  • Its cariostatic effects are partly attributed to antimicrobial activity, but the precise impact on oral microbiota is not fully understood.

Purpose of the Study:

  • To investigate the mechanisms by which SDF affects the oral microbiota.
  • To compare the microbial composition of carious lesions before and after SDF treatment.

Main Methods:

  • A case-control study involving primary teeth with carious lesions.
  • Microbial viability testing and 16S rRNA gene sequencing were used to analyze surface biofilm and subsurface dentin.
  • Samples were compared between untreated and SDF-treated lesions.

Main Results:

  • SDF treatment did not significantly alter microbial viability or community composition in the carious surface biofilm.
  • However, significant differences in microbial community composition were observed in subsurface carious dentin between SDF-treated and untreated lesions.
  • The abundance of 15 bacterial species differed significantly in subsurface dentin post-SDF treatment.

Conclusions:

  • SDF does not significantly change the microbial community of the carious surface biofilm.
  • SDF induces a shift in microbial community membership within dentin tubules.
  • This represents a novel mechanism of action for SDF in arresting dental caries.