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A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
Published on: June 15, 2011
The association of the TLR3 SNP rs3775291 with schizophrenia is influenced by age
Yulong Wang1, Lin Peng2, Yue Liu3
1College of Teacher Education, Qilu Normal University, Jinan, Shandong, China.
Background:
Toll-like receptor 3 (TLR3) plays an important role in the pathogenesis of schizophrenia and the TLR3 single nucleotide polymorphism (SNP) rs3775291 is associated with central nervous system diseases. In this study, we analysed the association between rs3775291 and schizophrenia.
Methods:
A case-control study was performed that included 394 patients with schizophrenia and 445 healthy controls. Peripheral venous blood was collected, whole-genome DNA was extracted, and rs3775291 polymorphisms were detected using PCR and direct sequencing. Differences in distribution of allele and genotype frequencies between the two groups were compared using co-dominant, dominant, and recessive genetic models. The effects of sex, age, and family history were also analysed.
Results:
Allele and genotype frequencies of rs3775291 were not associated with schizophrenia overall, nor were they associated with schizophrenia in the different sex or family history groups (P > 0.05). However, in the < 40 years age group, co-dominant and dominant model analyses showed that the frequency of TC and CC genotypes in patients with schizophrenia was significantly higher than that in the control group (P < 0.05), and TC and CC genotypes were associated with susceptibility to schizophrenia. There was no significant difference among people older than 40 years.
Conclusion:
The effect of rs3775291 on schizophrenia was age-related, TC and CC genotypes were associated with susceptibility to schizophrenia in younger individuals, and the TT genotype had a protective effect regarding schizophrenia.
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