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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jun 5, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Characterization Standard for In-situ Cryo-electron Tomography.

Mallak Ali1, Joshua Hutchings1, Tahiti Dutta2

  • 1Biohub, Redwood City, CA 94063, USA.

Biorxiv : the Preprint Server for Biology
|June 4, 2026
PubMed
Summary
This summary is machine-generated.

PP7 virus-like particles (VLPs) offer a new standard for cryo-electron tomography (cryo-ET) workflows. These particles enable rapid, high-resolution validation of cryo-ET pipelines using smaller datasets.

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Last Updated: Jun 5, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Published on: July 12, 2022

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Area of Science:

  • Structural Biology
  • Microscopy Techniques
  • Biophysics

Background:

  • Standardized biological specimens are crucial for optimizing cryo-electron microscopy (cryoEM) workflows and benchmarking instrument performance.
  • Apoferritin serves as a standard for single-particle analysis, but a similar benchmark is lacking for cryo-electron tomography (cryo-ET).
  • Ribosomes are commonly used in cryo-ET but require large datasets due to C1 symmetry and structural heterogeneity, hindering rapid optimization and standardized comparisons.

Purpose of the Study:

  • To introduce a scalable in situ benchmark for cryo-electron tomography (cryo-ET).
  • To provide a standardized specimen for optimizing cryo-ET workflows and validating instrument performance.

Main Methods:

  • Overexpression of PP7 virus-like particles (VLPs) in *E. coli*.
  • Utilizing the high symmetry of VLPs for rapid validation.
  • Employing distinct structural features of VLPs as a resolution metric.

Main Results:

  • PP7 VLPs serve as a scalable in situ benchmark for cryo-ET.
  • High symmetry of VLPs allows for high-resolution validation of tomographic pipelines with minimal datasets.
  • Distinct structural features provide a practical resolution metric across various resolutions.

Conclusions:

  • PP7 VLPs are a suitable replacement for ribosomes as a standard in cryo-ET.
  • This VLP benchmark facilitates rapid optimization and standardized comparison of cryo-ET workflows.
  • The use of PP7 VLPs can accelerate advancements in cryo-ET methodology and application.