Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Grooming as a window into the post-stress recuperative process.

bioRxiv : the preprint server for biology·2026
Same author

State-dependent geometric transformations in the mouse hippocampus support fear generalization without loss of discriminability.

bioRxiv : the preprint server for biology·2026
Same author

VesiclePy: A machine learning vesicle analysis toolbox for volume electron microscopy.

PLoS computational biology·2026
Same author

GloBIAS: strengthening the foundations of bioimage analysis.

Nature methods·2026
Same author

NucleoNet and DropNet: Generalist deep learning models for instance segmentation of nuclei and lipid droplets from electron microscopy images.

bioRxiv : the preprint server for biology·2026
Same author

A machine learning approach to using ultrasound for body composition and nutritional status assessment in newborns: a pilot study protocol.

Pilot and feasibility studies·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Simultaneous brain-wide single-cell recording resolves spatiotemporal memory architecture.

Dongqing Shi1,2, Yongjie Hou1,2, Yixiao Yan1,2

  • 1Department of Cell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI, USA.

Biorxiv : the Preprint Server for Biology
|June 4, 2026
PubMed
Summary
This summary is machine-generated.

GLOBE enables brain-wide, single-cell activity recording in vivo. This technology captures neural activity across large networks, advancing our understanding of brain functions and diseases.

More Related Videos

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously
06:07

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously

Published on: March 14, 2022

Recording and Analyzing Multimodal Large-Scale Neuronal Ensemble Dynamics on CMOS-Integrated High-Density Microelectrode Array
09:44

Recording and Analyzing Multimodal Large-Scale Neuronal Ensemble Dynamics on CMOS-Integrated High-Density Microelectrode Array

Published on: March 8, 2024

Related Experiment Videos

Last Updated: Jun 5, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously
06:07

Construction of Local Field Potential Microelectrodes for in vivo Recordings from Multiple Brain Structures Simultaneously

Published on: March 14, 2022

Recording and Analyzing Multimodal Large-Scale Neuronal Ensemble Dynamics on CMOS-Integrated High-Density Microelectrode Array
09:44

Recording and Analyzing Multimodal Large-Scale Neuronal Ensemble Dynamics on CMOS-Integrated High-Density Microelectrode Array

Published on: March 8, 2024

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Bioengineering

Background:

  • Mammalian brain functions rely on coordinated activity across large-scale neural networks.
  • Understanding these networks in health and disease requires simultaneous, brain-wide single-cell activity measurement.
  • Current live-measurement techniques face limitations in balancing spatial resolution and scale.

Purpose of the Study:

  • To introduce GLOBE (sinGle-cell spatiotemporaL recOrding Brain-widE), a novel technology for in vivo, brain-wide single-cell recording.
  • To demonstrate GLOBE's capability for high-resolution, sensitive, and scalable measurement of cellular activity.
  • To apply GLOBE to map brain-wide neural activity dynamics during fear learning and memory retrieval.

Main Methods:

  • GLOBE utilizes genetically encoded intracellular protein tape recorders for analog signal recording.
  • A high-throughput computational platform integrates image and signal analysis for accelerated readout.
  • Standard light microscopy is used for in situ readout, compatible with expansion microscopy and RNA analysis.

Main Results:

  • GLOBE recorded Fos transcriptional activity in up to 219,703 neurons across a mouse brain over 5.5 days.
  • Achieved timestamp precision of 3.1-6.7 hours and high local recording density (69-90% of neurons per field of view).
  • Revealed brain-wide spatiotemporal structure of Fos activity, showing region-specific temporal heterogeneity in fear learning and memory retrieval.

Conclusions:

  • GLOBE provides unprecedented spatiotemporal resolution for brain-wide single-cell activity recording.
  • The technology resolves distributed neural dynamics, with variance scaling down as cell numbers increase.
  • GLOBE offers broad applications for dissecting physiological and pathological processes at the brain-wide scale.