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Related Experiment Video

Updated: Jun 5, 2026

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Published on: May 12, 2017

TSC2 is a stress granule suppressor.

Yizhe Ma¹, Natalie G Farny^1,2

¹Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.

Biorxiv : the Preprint Server for Biology

|June 4, 2026

Summary

Tsc2 loss causes spontaneous stress granules (SGs) via mTORC1. Reduced Tsc2 levels increase stress sensitivity, highlighting Tsc2

Keywords:

RNAi screen Stress granules translational control tuberous sclerosis complex

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Measurements of Physiological Stress Responses in C. Elegans

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Published on: May 21, 2020

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Last Updated: Jun 5, 2026

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Published on: May 12, 2017

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Stress granules (SGs) are dynamic ribonucleoprotein assemblies crucial for cellular proteostasis during stress.
Liquid-liquid phase separation drives SG formation, enabling cells to survive adverse conditions.

Purpose of the Study:

To identify novel conserved regulators of stress granule dynamics.
To investigate the role of Tsc2 in stress granule formation and clearance.

Main Methods:

Reanalysis of a *Drosophila* genome-wide RNAi screen to identify SG suppressor genes.
Validation of Tsc2 in mouse embryonic fibroblasts (MEFs) and human cell lines.
Assessment of SG formation, clearance, and cellular stress responses in Tsc2-deficient models.

Main Results:

Complete loss of Tsc2 in MEFs induced spontaneous, translation-dependent SGs, linked to mTORC1 hyperactivation.
Tsc2-deficient MEFs exhibited increased sensitivity to endoplasmic reticulum stress, with delayed SG clearance.
Partial TSC2 reduction in human cells did not cause spontaneous SGs but correlated stress sensitivity and clearance delays with remaining TSC2 levels.

Conclusions:

TSC2 acts as a threshold-dependent regulator of SG assembly.
Tsc2 is a key regulator of stress granule dynamics, influencing cellular responses to various stressors.