Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Local Sodium Tanshinone IIA Sulfonate Attenuates Periodontal Bone Loss by Modulating Inflammatory, Immune and Osteoclastogenic Signalling.

Journal of clinical periodontology·2026
Same author

Interaction of Hypoxia and Kynurenine Pathway in Periodontal Inflammation.

International journal of tryptophan research : IJTR·2026
Same author

Preparation and In Vitro Characterization of Quercetin and Chlorhexidine-loaded PLGA Nanoparticles for Oral Biofilm-Associated Pathologies.

Clinical oral investigations·2026
Same author

Profiling Gingival Inflammation in a 3D Oral Tissue Model Reveals Early Features of Disease Progression.

bioRxiv : the preprint server for biology·2026
Same author

Oncofetal RNA-binding proteins of IGF2BP family suppress IRF-3 and NF-kB dependent transcription downstream of cytosolic RNA sensors.

bioRxiv : the preprint server for biology·2026
Same author

Porphyromonas gingivalis lipopolysaccharide promotes peri-implant inflammation through neutrophil activation.

Journal of prosthodontic research·2026

Related Experiment Video

Updated: Jun 5, 2026

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

Estrogen Deprivation and Periodontitis Interact Across Multiple Tissues.

Nil Yakar1, Hatice Hasturk1, Carla Alvarez Rivas2

  • 1ADA Forsyth Institute, Somerville, MA.

Biorxiv : the Preprint Server for Biology
|June 4, 2026
PubMed
Summary

Estrogen deprivation worsens periodontitis-related bone loss and alters immune cell function. Estrogen replacement partially restores gene expression in affected tissues, highlighting its protective role.

Related Experiment Videos

Last Updated: Jun 5, 2026

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

Area of Science:

  • Oral biology and immunology
  • Endocrinology and bone metabolism
  • Microbiome research

Background:

  • Estrogen plays a crucial role in maintaining bone health and immune homeostasis.
  • Periodontitis, a chronic inflammatory disease, affects periodontal tissues and can have systemic implications.
  • The interplay between estrogen deficiency and periodontitis is not fully understood, particularly concerning bone marrow and local tissue responses.

Purpose of the Study:

  • To investigate the combined effects of estrogen deprivation and ligature-induced periodontitis (LIP) on bone marrow and periodontal tissues.
  • To analyze immune cell function and gene expression changes in response to these conditions.
  • To evaluate the impact of estrogen replacement therapy on these alterations.

Main Methods:

  • A mouse model combining ovariectomy-induced estrogen deprivation with LIP was employed.
  • Bone marrow myeloid cell proportions were assessed via flow cytometry.
  • Femur metaphysis was analyzed using histology and micro-CT.
  • Ex vivo cytokine responses of myeloid cells to lipopolysaccharide (LPS) were measured.
  • Microbiome composition and gingival/dentoalveolar transcriptomic changes were analyzed using 16S rRNA and bulk RNA sequencing, respectively.

Main Results:

  • Ovariectomy increased osteoblast-like and adipocyte-like cells in bone marrow, while LIP decreased them.
  • Estrogen deprivation exacerbated LIP-induced alveolar bone loss, increasing osteoclast numbers.
  • Bone marrow myeloid cells from ovariectomized mice showed reduced expression of TNFα, CCL2, and IL10 upon LPS stimulation.
  • Transcriptomic analysis revealed estrogen-responsive gene expression changes in gingival and dentoalveolar tissues during periodontitis.

Conclusions:

  • Estrogen deprivation and periodontitis synergistically impact the bone marrow niche and exacerbate alveolar bone loss.
  • Estrogen deficiency modulates immune cell function and alters the expression of key genes involved in immunity and tissue regulation in periodontal tissues.
  • Estrogen replacement therapy may partially reverse these detrimental effects, underscoring estrogen's protective role in periodontitis.